N-glycosylation is involved in the sensitivity of Saccharomyces cerevisiae to HM-1 killer toxin secreted from Hansenula mrakii IFO 0895.
Appl Microbiol Biotechnol
; 51(2): 176-84, 1999 Feb.
Article
en En
| MEDLINE
| ID: mdl-10091323
Saccharomyces cerevisiae rhk mutants were previously shown to have a phenotype that is resistant to HM-1 killer toxin secreted from Hansenula mrakii IFO 0895. The RHK1/ALG3 gene encodes a mannosyl-transferase that is involved in the synthesis of an oligosaccharide in protein N-glycosylation. Previously, this gene was cloned and shown to complement the rhk1 mutation. In this study, the RHK2 gene, which complements the rhk2 mutation, was cloned. The RHK2 gene was found to be identical to the essential gene STT3, which encodes a subunit of the oligosaccharyl-transferase complex. This complex transfers the core oligosaccharide to proteins. The rhk2 mutants showed supersensitivity to several drugs (Calcofluor White, caffeine and FK506), suggesting that these strains have cell-wall defects. Activity staining of invertase in an acrylamide gel indicated that it was underglycosylated. These results suggest that one or more mannoproteins are involved in the cytocidal process of HM-1.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pichia
/
Saccharomyces cerevisiae
/
Proteínas de Saccharomyces cerevisiae
/
Micotoxinas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Año:
1999
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Alemania