Crystallization and preliminary X-ray crystallographic analysis of the unusual ferritin from Listeria innocua.

Ilari, A; Savino, C; Stefanini, S; Chiancone, E; Tsernoglou, D

Ilari, A; Savino, C; Stefanini, S; Chiancone, E; Tsernoglou, D.

Afiliación

Ilari A; CNR Center of Molecular Biology, Department of Biochemical Sciences 'A. Rossi Fanelli', University of Rome 'La Sapienza', Piazzale Aldo Moro, 5, 00185 Rome, Italy.

Acta Crystallogr D Biol Crystallogr ; 55(Pt 2): 552-3, 1999 Feb.

Article en En | MEDLINE | ID: mdl-10089376

RESUMEN

Single crystals of ferritin extracted from Listeria innocua have been obtained by the vapour-diffusion method using PEG 1000 as precipitant. The crystals are orthorhombic, space group P212121, with unit-cell dimensions a = 87.7, b = 137.5, c = 173.1 A. The crystals diffract to 2.9 A resolution on a rotating-anode X-ray source and to 2.35 A resolution on a synchrotron X-ray source. The asymmetric unit contains one molecule formed by 12 subunits, corresponding to a packing density of 2.41 A3 Da-1

Asunto(s)

Ferritinas/química; Listeria/química; Cristalización; Cristalografía por Rayos X; Conformación Proteica

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ferritinas / Listeria Idioma: En Revista: Acta Crystallogr D Biol Crystallogr Año: 1999 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos

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