Surfaces designed to control the projected area and shape of individual cells.
J Biomech Eng
; 121(1): 40-8, 1999 Feb.
Article
en En
| MEDLINE
| ID: mdl-10080088
Materials with spatially resolved surface chemistry were designed to isolate individual mammalian cells to determine the influence of projected area on specific cell functions (e.g., proliferation, cytoskeletal organization). Surfaces were fabricated using a photolithographic process resulting in islands of cell binding N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane (EDS) separated by a nonadhesive interpenetrating polymer network [poly (acrylamide-co-ethylene glycol); P (AAm-co-EG)]. The surfaces contained over 3800 adhesive islands/cm2, allowing for isolation of single cells with projected areas ranging from 100 microns 2 to 10,000 microns 2. These surfaces provide a useful tool for researching how cell morphology and mechanical forces affect cell function.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Propiedades de Superficie
/
Materiales Biocompatibles
/
Técnicas de Cultivo de Célula
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Biomech Eng
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos