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Genetic factors influence cataract formation in alpha 3 connexin knockout mice.
Gong, X; Agopian, K; Kumar, N M; Gilula, N B.
Afiliación
  • Gong X; Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037, USA.
Dev Genet ; 24(1-2): 27-32, 1999.
Article en En | MEDLINE | ID: mdl-10079508
Connexin alpha 3 (Cx46 or Gja3) gene targeted null mice developed lens nuclear cataracts shortly after birth. A large variance in the cataracts was observed in alpha 3 null sibs on a mixed 129SvJae X C57BL/6J F3 background. This suggested that the genetic background might influence the cataract phenotype. Therefore, we placed the alpha 3 null mutation into a 129SvJae background, and also backcrossed the mutation for six generations into 129SvJ and C57BL/6J backgrounds. While alpha 3 nulls on the two 129 backgrounds contained severe cataracts associated with gamma crystallin cleavage, alpha 3 nulls on the C57B16 background had far milder cataracts with no detectable gamma crystallin cleavage. These findings suggest that a genetic modifier exists that influences gamma crystallin stability, and that gamma crystallin breakdown is associated with severe nuclear cataracts.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Catarata / Conexinas / Cristalinas / Cristalino Límite: Animals Idioma: En Revista: Dev Genet Año: 1999 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Catarata / Conexinas / Cristalinas / Cristalino Límite: Animals Idioma: En Revista: Dev Genet Año: 1999 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos