High-resolution, human-bovine comparative mapping based on a closed YAC contig spanning the bovine mh locus.
Mamm Genome
; 10(3): 289-93, 1999 Mar.
Article
en En
| MEDLINE
| ID: mdl-10051326
A closed YAC contig spanning the mh locus was assembled by STS content mapping with seven microsatellite markers, eight genes or EST, and nine STS corresponding to YAC ends. The contig comprises 27 YACs, has an average depth of 4.3 YACs, and spans an estimated 1.2 Mb. A linkage map was constructed based on five of the microsatellite markers anchored to the contig and shown to span 7 cM, yielding a ratio of 160 kb/1 cM for the corresponding chromosome region. Comparative mapping data indicate that the constructed contig spans an evolutionary breakpoint connecting two chromosome segments that are syntenic but not adjacent in the human. Consolidation of human gene order by means of whole genome radiation hybrids and its comparison with the bovine order as inferred from the contig confirm conservation of gene order within segments.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factor de Crecimiento Transformador beta
/
Mapeo Contig
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Mamm Genome
Asunto de la revista:
GENETICA
Año:
1999
Tipo del documento:
Article
País de afiliación:
Bélgica
Pais de publicación:
Estados Unidos