Detection of Leishmania (Viannia) braziliensis by PCR in samples collected by scraping the lesion edges from patients of an endemic area
Rev. Inst. Adolfo Lutz (Online)
; 74(4): 437-441, out.-dez.2015. ilus, tab
Article
en En
| LILACS, SES-SP
| ID: lil-797169
Biblioteca responsable:
BR91.2
ABSTRACT
American cutaneous leishmaniasis (ACL) is an infectious disease caused by Leishmania. Their diagnosis is performed in samples collected from the lesion biopsies, which has to be performedby physicians. For simplifying the sample collection, this study proposes a minimally invasive procedure, by scraping the lesion edges. Laboratory diagnosis by PCR was performed and compared with the microscopic examination, by analyzing 28 samples collected from patients with suspicion of ACL. Sample collected from the lesion edge with a sterile toothpick was divided into two aliquots. One aliquot was analyzed under direct microscopy, and the second by PCR, by using two primer pairs (one for Leishmania genus and other for L. (V.) braziliensis). Of 28 samples, 27 (96.43 %) showed concordant results in both methodologies (eight positive and 20 negative). The PCR methodologyis an invaluable tool (i) to determine the Leishmania species; (ii) to provide an alternative procedure of sample collection, when an authorized professional is not available in the respective health service;and (iii) to propose a minimally invasive procedure for collecting biological material...
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
/
SES-SP
Asunto principal:
Leishmania braziliensis
/
Reacción en Cadena de la Polimerasa
/
Leishmaniasis Cutánea
Tipo de estudio:
Diagnostic_studies
Límite:
Female
/
Humans
/
Male
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Rev. Inst. Adolfo Lutz (Online)
Año:
2015
Tipo del documento:
Article
País de afiliación:
Brasil