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The expression of heterologous MAM-7 in Lactobacillus rhamnosus reduces its intrinsic capacity to inhibit colonization of pathogen Vibrio parahaemolyticus in vitro
Beltran, Sebastian; Munoz-Bergmann, Cristian A; Elola-Lopez, Ana; Quintana, Javiera; Segovia, Cristopher; Trombert, Annette N.
Afiliación
  • Beltran, Sebastian; Universidad Mayor. Facultad de Medicina. Escuela de Tecnologia Médica. Santiago. CL
  • Munoz-Bergmann, Cristian A; Universidad Mayor. Facultad de Medicina. Escuela de Tecnologia Médica. Santiago. CL
  • Elola-Lopez, Ana; Universidad Mayor. Facultad de Medicina. Escuela de Tecnologia Médica. Santiago. CL
  • Quintana, Javiera; Universidad Mayor. Facultad de Medicina. Escuela de Tecnologia Médica. Santiago. CL
  • Segovia, Cristopher; Universidad Mayor. Facultad de Medicina. Escuela de Tecnologia Médica. Santiago. CL
  • Trombert, Annette N; Universidad Mayor. Facultad de Medicina. Escuela de Tecnologia Médica. Santiago. CL
Biol. Res ; 49: 1-10, 2016. ilus, graf
Article en En | LILACS | ID: lil-774429
Biblioteca responsable: CL1.1
ABSTRACT

BACKGROUND:

Vibrio parahaemolyticus (V. parahaemolyticus) is a Gram-negative, halophilic bacterium recognized as one of the most important foodborne pathogen. When ingested, V. parahaemolyticus causes a self-limiting illness (Vibriosis), characterized mainly by watery diarrhoea. Treatment is usually oral rehydration and/or antibiotics in complicated cases. Since 1996, the pathogenic and pandemic V. parahaemolyticus O3K6 serotype has spread worldwide, increasing the reported number of vibriosis cases. Thus, the design of new strategies for pathogen control and illness prevention is necessary. Lactobacillus sp. grouped Gram positive innocuous bacteria, part of normal intestinal microbiota and usually used as oral vaccines for several diarrheic diseases. Recombinants strains of Lactobacillus (RL) expressing pathogen antigens can be used as part of an anti-adhesion strategy where RL block the pathogen union sites in host cells. Thus, we aimed to express MAM-7 V. parahaemolyticus adhesion protein in Lactobacillus sp. to generate an RL that prevents pathogen colonization

RESULTS:

We cloned the MAM-7 gene from V. parahaemolyticus RIMD 2210633 in Lactobacillus expression vectors. Recombinant strains (Lactobacillus rhamnosus pSEC-MAM7 and L. rhamnosus pCWA-MAM7) adhered to CaCo-2 cells and competed with the pathogen. However, the L. rhamnosus wild type strain showed the best capacity to inhibit pathogen colonization in vitro. In addition, LDH-assay showed that recombinant strains were cytotoxic compared with the wild type isogenic strain

CONCLUSIONS:

MAM-7 expression in lactobacilli reduces the intrinsic inhibitory capacity of L. rhamnosus against V. parahaemolyticus.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Vibrio parahaemolyticus / Adhesión Bacteriana / Adhesinas Bacterianas / Lacticaseibacillus rhamnosus Tipo de estudio: Evaluation_studies Límite: Humans Idioma: En Revista: Biol. Res Asunto de la revista: BIOLOGIA Año: 2016 Tipo del documento: Article / Project document País de afiliación: Chile Pais de publicación: Chile
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Vibrio parahaemolyticus / Adhesión Bacteriana / Adhesinas Bacterianas / Lacticaseibacillus rhamnosus Tipo de estudio: Evaluation_studies Límite: Humans Idioma: En Revista: Biol. Res Asunto de la revista: BIOLOGIA Año: 2016 Tipo del documento: Article / Project document País de afiliación: Chile Pais de publicación: Chile