Your browser doesn't support javascript.
loading
Multiple gene sequence analysis using genes of the bacterial DNA repair pathway
Rotelok Neto, Miguel; Galvão, Carolina Weigert; Cruz, Leonardo Magalhães; Guizelini, Dieval; Silva, Leilane Caline; Garcia, Jarem Raul; Etto, Rafael Mazer.
Afiliación
  • Rotelok Neto, Miguel; Universidade Estadual de Ponta Grossa. Departamento de Biologia Estrutural, Molecular e Genética. Ponta Grossa. BR
  • Galvão, Carolina Weigert; Universidade Estadual de Ponta Grossa. Departamento de Biologia Estrutural, Molecular e Genética. Ponta Grossa. BR
  • Cruz, Leonardo Magalhães; Universidade Estadual de Ponta Grossa. Departamento de Biologia Estrutural, Molecular e Genética. Ponta Grossa. BR
  • Guizelini, Dieval; Universidade Estadual de Ponta Grossa. Departamento de Biologia Estrutural, Molecular e Genética. Ponta Grossa. BR
  • Silva, Leilane Caline; Universidade Estadual de Ponta Grossa. Departamento de Biologia Estrutural, Molecular e Genética. Ponta Grossa. BR
  • Garcia, Jarem Raul; Universidade Estadual de Ponta Grossa. Departamento de Biologia Estrutural, Molecular e Genética. Ponta Grossa. BR
  • Etto, Rafael Mazer; Universidade Estadual de Ponta Grossa. Departamento de Biologia Estrutural, Molecular e Genética. Ponta Grossa. BR
Braz. arch. biol. technol ; 58(3): 421-430, May-Jun/2015. tab, graf
Article en En | LILACS | ID: lil-748198
Biblioteca responsable: BR1.1
ABSTRACT
The ability to recognize and repair abnormal DNA structures is common to all forms of life. Physiological studies and genomic sequencing of a variety of bacterial species have identified an incredible diversity of DNA repair pathways. Despite the amount of available genes in public database, the usual method to place genomes in a taxonomic context is based mainly on the 16S rRNA or housekeeping genes. Thus, the relationships among genomes remain poorly understood. In this work, an approach of multiple gene sequence analysis based on genes of DNA repair pathway was used to compare bacterial genomes. Housekeeping and DNA repair genes were searched in 872 completely sequenced bacterial genomes. Seven DNA repair and housekeeping genes from distinct metabolic pathways were selected, aligned, edited and concatenated head-to-tail to form a super-gene. Results showed that the multiple gene sequence analysis using DNA repair genes had better resolution at class level than the housekeeping genes. As housekeeping genes, the DNA repair genes were advantageous to separate bacterial groups at low taxonomic levels and also sensitive to genes derived from horizontal transfer.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Idioma: En Revista: Braz. arch. biol. technol Asunto de la revista: BIOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Idioma: En Revista: Braz. arch. biol. technol Asunto de la revista: BIOLOGIA Año: 2015 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil