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Evaluation of the level of microbial contamination and prevalence of gram-negative non-fermentative rods in dental unit waterlines
Kotaka, Cinthia Regiane; Garcia, Lourdes Botelho; Ito, Fernanda Akemi Nakanishi; Fuganti, Marcel Rodrigo; Carnio, João; Pelayo, Jacinta Sanchez.
Afiliación
  • Kotaka, Cinthia Regiane; State University of Londrina. Department of Microbiology. Londrina. BR
  • Garcia, Lourdes Botelho; State University of Londrina. Department of Microbiology. Londrina. BR
  • Ito, Fernanda Akemi Nakanishi; State University of Londrina. Department of Microbiology. Londrina. BR
  • Fuganti, Marcel Rodrigo; State University of Londrina. Department of Microbiology. Londrina. BR
  • Carnio, João; State University of Londrina. Department of Microbiology. Londrina. BR
  • Pelayo, Jacinta Sanchez; State University of Londrina. Department of Microbiology. Londrina. BR
RSBO (Impr.) ; 9(3): 245-253, Jul.-Sep. 2012. tab
Article en En | LILACS | ID: lil-748120
Biblioteca responsable: BR97.1
ABSTRACT

Introduction:

The cross infection control in dental office has received great attention from professionals and one of the critical points is the bacteriological control of water used in dental unit.

Objective:

To perform a microbiological evaluation of the water used in dental units, the identification of Gram-negative non-fermentative rods (GNNR) and their ability to adhere to polystyrene, and the antimicrobial activity of disinfectants on the identified strains. Material and

methods:

The heterotrophic bacteria count and GNNR identification were performed on water samples collected from 25 dental units (air/water syringe and reservoir). The GNNR were assessed on their capability to adhere to polystyrene and on their antimicrobial activity to the following disinfectants sodium hypochlorite (0.06%, 0.12%, 0.25%, and0.5%) and chlorhexidine (0.03%, 0.06%, and 0.12%).

Results:

88% of the air/water syringe collected samples and 68% of the reservoir collected samples were out of the potability standards. The quantity of isolated bacteria from the reservoir was lower than from the air/water syringe in 88% of the dental units. Methylobacterium spp. was found in highest percentage (19.7%) during GNNR genus isolation. There was a weak adherence to polystyrene in 85.04% of the samples. Sodium hypochlorite at 0.25%, inactivated 100% of the GNNRs in 10 minutes, while the highest tested concentration of chlorhexidine (0.12%), inactivated 98.5% of the GNNRs.

Conclusion:

These results provide information on the contamination problem of dental unit waterlines (DUWL) and indicate a need for treatment of the water used in dental units. The disinfection of DUWL can be performed with sodium hypochlorite at 0.25% (half the concentration recommended in the literature). However, further studies are necessary regarding DUWL frequency disinfection.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Tipo de estudio: Guideline / Prevalence_studies / Prognostic_studies / Risk_factors_studies Idioma: En Revista: RSBO (Impr.) Asunto de la revista: ODONTOLOGIA Año: 2012 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Tipo de estudio: Guideline / Prevalence_studies / Prognostic_studies / Risk_factors_studies Idioma: En Revista: RSBO (Impr.) Asunto de la revista: ODONTOLOGIA Año: 2012 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil