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Ardra profiles of bacteria and archaea in mangrove sediments with different levels of contamination in the estuarine complex of Paranaguá, Brazil
Ribeiro, Catherine Gérikas; Steffens, Maria Berenice Reynaud; Etto, Rafael Mazer; Galvão, Carolina Weigert; Martins, César de Castro; Pedrosa, Fábio de Oliveira; Kolm, Hedda Elisabeth.
Afiliación
  • Ribeiro, Catherine Gérikas; Universidade de São Paulo. Instituto Oceanográfico. Laboratório de Biogeoquímica Marinha. São Paulo. BR
  • Steffens, Maria Berenice Reynaud; Universidade de São Paulo. Instituto Oceanográfico. Laboratório de Biogeoquímica Marinha. São Paulo. BR
  • Etto, Rafael Mazer; Universidade de São Paulo. Instituto Oceanográfico. Laboratório de Biogeoquímica Marinha. São Paulo. BR
  • Galvão, Carolina Weigert; Universidade de São Paulo. Instituto Oceanográfico. Laboratório de Biogeoquímica Marinha. São Paulo. BR
  • Martins, César de Castro; Universidade de São Paulo. Instituto Oceanográfico. Laboratório de Biogeoquímica Marinha. São Paulo. BR
  • Pedrosa, Fábio de Oliveira; Universidade de São Paulo. Instituto Oceanográfico. Laboratório de Biogeoquímica Marinha. São Paulo. BR
  • Kolm, Hedda Elisabeth; Universidade de São Paulo. Instituto Oceanográfico. Laboratório de Biogeoquímica Marinha. São Paulo. BR
Braz. arch. biol. technol ; Braz. arch. biol. technol;56(2): 275-281, Mar.-Apr. 2013. ilus, graf, mapas, tab
Article en En | LILACS | ID: lil-675646
Biblioteca responsable: BR1.1
ABSTRACT
The mangrove's sediments from the coastal areas under human activities may contain significant contaminations by hydrocarbons, even when there are no visual evidences of it. The microorganisms are essential to these ecosystems, especially in the control of their chemical environment. Sediment samples were collected in two regions under different environment conditions (pristine and contaminated) of the Paranaguá Estuarine Complex (Paranaguá Bay and Laranjeiras Bay), Brazil. Aliphatic hydrocarbons were determined by the GC-FID to assess the status of contamination of the studied areas. The total DNA was extracted from these samples. The 16S rRNA gene was amplified by the PCR reactions with the pair of primers 21F and 958R for the archaeal domain, and 27F and 1492R for the bacterial domain. Comparisons of communities were made by the ARDRA technique, using the HinfI restriction enzyme. The phosphate concentration showed significant differences between the two regions. The aliphatic hydrocarbons analysis showed the presence of unresolved complex mixture (UCM), an indicator of oil contamination, in the samples from the Paranaguá Bay, which was corroborated by the concentration of total aliphatic hydrocarbons. The ARDRA profile indicated that the structure of archaeal and bacterial communities of the sampled areas was very similar. Therefore, the anthropogenic influences in the Paranaguá Bay showed to be not sufficient to produce disturbances in the prokaryotic dominant groups.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS País/Región como asunto: America do sul / Brasil Idioma: En Revista: Braz. arch. biol. technol Asunto de la revista: BIOLOGIA Año: 2013 Tipo del documento: Article / Project document País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS País/Región como asunto: America do sul / Brasil Idioma: En Revista: Braz. arch. biol. technol Asunto de la revista: BIOLOGIA Año: 2013 Tipo del documento: Article / Project document País de afiliación: Brasil Pais de publicación: Brasil