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Rotavirus infection in a tertiary hospital: laboratory diagnosis and impact of immunization on pediatric hospitalization
Pereira, Luciane Aparecida; Raboni, Sonia Mara; Nogueira, Meri B; Vidal, Luine R; Almeida, Sergio Monteiro de; Debur, Maria C; Cruz, Cristina.
Afiliación
  • Pereira, Luciane Aparecida; Universidade Federal do Paraná. Laboratory of Virology. BR
  • Raboni, Sonia Mara; Universidade Federal do Paraná. Infectious Diseases Departament. BR
  • Nogueira, Meri B; Universidade Federal do Paraná. Laboratory of Virology. BR
  • Vidal, Luine R; Universidade Federal do Paraná. Laboratory of Virology. BR
  • Almeida, Sergio Monteiro de; Universidade Federal do Paraná. Laboratory of Virology. BR
  • Debur, Maria C; Secretaria Estadual de Saúde do Paraná. Central Laboratory of Public Health. BR
  • Cruz, Cristina; Universidade Federal do Paraná. BR
Braz. j. infect. dis ; 15(3): 215-219, May-June 2011. ilus
Article en En | LILACS | ID: lil-589951
Biblioteca responsable: BR1.1
ABSTRACT
BACKGROUND AND

OBJECTIVES:

Rotavirus (RV) is the main etiological agent of diarrhea in childhood; its laboratory diagnosis is crucial to guide the clinical management and prevention of its spread. RV immunization was introduced in Brazilian 6-month-old children in 2006. The present study was aimed to evaluate three methodologies used for human RV detection in stool samples obtained from patients hospitalized due to gastroenteritis in a teaching hospital and report the impact of RV immunization in hospitalization by diarrhea.

METHODS:

293 stool samples collected in the 2001-2008 period were analyzed by enzyme immunoassay (EIA), latex agglutination (LA) and polyacrylamide gel electrophoresis (PAGE).

RESULTS:

Rotavirus was detected in 34.8 percent of samples by LA assay, 28.3 percent of samples by EIA assay and in 25.6 percent of samples by PAGE assay. Considering the PAGE method as gold standard, the sensitivity, specificity and accuracy of EIA were 94.6 percent, 94.4 percent and 94.5 percent, and to LA were 82.6 percent, 81.6 percent and 81.9 percent, respectively.

CONCLUSION:

These results indicate that antigen detection by EIA is a rapid, sensitive and specific method, and could be used in large-scale applications for screening stool samples suspected of RV infection. This study showed decreased incidence of RV infection in hospitalized children prior to the implementation of the national immunization program against RV.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Infecciones por Rotavirus / Rotavirus / Vacunas contra Rotavirus / Diarrea / Heces / Gastroenteritis Tipo de estudio: Diagnostic_studies / Evaluation_studies / Incidence_studies / Prognostic_studies Límite: Child / Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Braz. j. infect. dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2011 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Infecciones por Rotavirus / Rotavirus / Vacunas contra Rotavirus / Diarrea / Heces / Gastroenteritis Tipo de estudio: Diagnostic_studies / Evaluation_studies / Incidence_studies / Prognostic_studies Límite: Child / Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Braz. j. infect. dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2011 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil