Your browser doesn't support javascript.
loading
Antimicrobial activity of an L-amino acid oxidase isolated from Bothrops leucurus snake venom
Torres, A. F. C; Dantas, R. T; Menezes, R. R. P. P. B; Toyama, M. H; Filho, E. D; Oliveira, M. F; Nogueira, N. A. P; Oliveira, M. R; Monteiro, H. S. A; Martins, A. M. C.
Afiliación
  • Torres, A. F. C; Federal University of Ceará. Department of Clinical and Toxicological Analysis. Fortaleza. BR
  • Dantas, R. T; Federal University of Ceará. Department of Physiology and Pharmacology. Fortaleza. BR
  • Menezes, R. R. P. P. B; Federal University of Ceará. Department of Clinical and Toxicological Analysis. Fortaleza. BR
  • Toyama, M. H; Universidade Estadual Paulista. São Vicente. BR
  • Filho, E. D; State University of Campinas. Institute of Biology. Campinas. BR
  • Oliveira, M. F; Federal University of Ceará. Department of Clinical and Toxicological Analysis. Fortaleza. BR
  • Nogueira, N. A. P; Federal University of Ceará. Department of Clinical and Toxicological Analysis. Fortaleza. BR
  • Oliveira, M. R; Federal University of Paraíba. Center of Exact Sciences and Nature. João Pessoa. BR
  • Monteiro, H. S. A; Federal University of Ceará. Department of Physiology and Pharmacology. Fortaleza. BR
  • Martins, A. M. C; Federal University of Ceará. Department of Clinical and Toxicological Analysis. Fortaleza. BR
J. venom. anim. toxins incl. trop. dis ; J. venom. anim. toxins incl. trop. dis;16(4): 614-622, 2010. ilus, graf
Article en En | LILACS | ID: lil-566161
Biblioteca responsable: BR33.1
ABSTRACT
Some snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW® (Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 ìg/mL, which corresponded to its minimum lethal concentration (MLC). BleuTV also inhibited the growth of promastigote forms of L. chagasi and L. amazonensis, with respective IC50 values of 1.94 ìg/mL and 5.49 ìg/mL. Furthermore, it repressed T. cruzi growth with an IC50 of 1.14 ìg/mL. However, BleuLAAO did not inhibit the growth of the microorganisms studied and was not toxic to macrophages. BleuTV had low toxicity against macrophages at the concentrations studied. In conclusion, whole venom from Bothrops leucurus inhibited the growth of some microorganisms, including S. aureus, Leishmania sp., and T. cruzi.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Venenos de Crotálidos / L-Aminoácido Oxidasa Límite: Animals Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Asunto de la revista: TOXICOLOGIA Año: 2010 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Venenos de Crotálidos / L-Aminoácido Oxidasa Límite: Animals Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Asunto de la revista: TOXICOLOGIA Año: 2010 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil