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Rapid detection of Vancomycin-Resistant Enterococci (VRE) in rectal samples from patients admitted to intensive care units
d'Azevedo, Pedro Alves; Santiago, Kelly Aline de Souza; Furtado, Guilherme Henrique Campos; Xavier, Diego Batista; Pignatari, Antonio Carlos Campos; Titze-de-Almeida, Ricardo.
Afiliación
  • d'Azevedo, Pedro Alves; Universidade Federal de São Paulo. Laboratório Especial de Microbiologia Clínica. São Paulo. BR
  • Santiago, Kelly Aline de Souza; Universidade Federal de São Paulo. Laboratório Especial de Microbiologia Clínica. São Paulo. BR
  • Furtado, Guilherme Henrique Campos; Universidade Federal de São Paulo. Comissão de Epidemiologia Hospitalar. São Paulo. BR
  • Xavier, Diego Batista; Universidade de Brasília. Laboratório de Microbiologia Molecular e Biotecnologia. Brasília. BR
  • Pignatari, Antonio Carlos Campos; Universidade Federal de São Paulo. Laboratório Especial de Microbiologia Clínica. São Paulo. BR
  • Titze-de-Almeida, Ricardo; Universidade de Brasília. Laboratório de Microbiologia Molecular e Biotecnologia. Brasília. BR
Braz. j. infect. dis ; Braz. j. infect. dis;13(4): 289-293, Aug. 2009. tab
Article en En | LILACS | ID: lil-539766
Biblioteca responsable: BR1.1
ABSTRACT
The reduction in time required to identify vancomycin-resistant enterococci (VRE) has gained increased importance during hospital outbreaks. In the present study, we implemented a laboratory protocol to speed up the VRE screening from rectal samples. The protocol combines a medium for selective VRE isolation (VREBAC®, Probac, São Paulo) and a multiplex PCR for detection and identification of vanA and vanB resistance genes. The screening performance was analyzed in 114 specimens collected from four intensive care units. The swabs were collected at two periods (1) during a VRE outbreak (February 2006, n=83 patients) and (2) at the post-outbreak period, after adoption of infection control measures (June 2006, n=31 patients). Forty-one/83 VRE (49.4 percent) and 3/31(9.7 percent) VRE were found at the first and second period, respectively. All isolates harbored the vanA gene. In both periods, detection of the gene vanA parallels to the minimum inhibitory concentration values of >256 µg/mL and >48 µg/mL for vancomycin and teicoplanin, respectively. Multiplex PCR and conventional methods agreed in 90.2 percent for enterococci identification. Besides this accuracy, we also found a remarkable reduction in time to obtain results. Detection of enterococcal species and identification of vancomycin resistance genes were ready in 29.5 hours, in comparison to 72 hours needed by the conventional methods. In conclusion, our protocol identified properly and rapidly enterococci species and vancomycin-resistance genes. The results strongly encourage its adoption by microbiology laboratories for VRE screenning in rectal samples.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Recto / Infección Hospitalaria / Brotes de Enfermedades / Enterococcus / Resistencia a la Vancomicina Tipo de estudio: Diagnostic_studies Límite: Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Braz. j. infect. dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2009 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Recto / Infección Hospitalaria / Brotes de Enfermedades / Enterococcus / Resistencia a la Vancomicina Tipo de estudio: Diagnostic_studies Límite: Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Braz. j. infect. dis Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2009 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil