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Human umbilical cord blood cells in infarcted rats
Pinho-Ribeiro, V; Maia, A. C. V; Werneck-de-Castro, J. P S; Oliveira, P. F; Goldenberg, R. C. S; Carvalho, A. C. Campos de.
Afiliación
  • Pinho-Ribeiro, V; Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro. BR
  • Maia, A. C. V; Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro. BR
  • Werneck-de-Castro, J. P S; Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro. BR
  • Oliveira, P. F; Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro. BR
  • Goldenberg, R. C. S; Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro. BR
  • Carvalho, A. C. Campos de; Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro. BR
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;43(3): 290-296, Mar. 2010. ilus, tab, graf
Article en En | LILACS | ID: lil-539715
Biblioteca responsable: BR1.1
ABSTRACT
Therapy with bone marrow-derived cells has been used in ischemic patients with reported success. The aim of this study was to determine the therapeutic efficacy of fresh and frozen human umbilical cord blood cells (hUCB) in Wistar rats submitted to permanent occlusion of the left coronary artery. Three hours after myocardial infarction, 2 x 10(7) hUCB cells or vehicle were administered by intramyocardial injection. The animals were divided into five groups control (N = 10), sham operated (N = 10), infarcted that received vehicle (N = 9), infarcted treated with cryopreserved hUCB (N = 7), and infarcted treated with fresh hUCB (N = 5). Cardiac function was evaluated by electrocardiogram (ECG) and echocardiogram (ECHO) before cell therapy, and by ECG, ECHO, cardiopulmonary test, and left ventricular pressure measurements 3 weeks later. After 3 weeks, both groups treated with hUCB still had Q wave present in L1, âQRS >90° and reduced shortening fraction (less than 50 percent). In addition, cardiac indexes of left ventricular contractility and relaxation were 5484 ± 875 and -4032 ± 643 mmHg (cryopreserved hUCB) and 4585 ± 955 and -2862 ± 590 mmHg (fresh hUCB), respectively. These values were not statistically different from those of saline-treated animals. Cardiopulmonary exercise test profile was typical of infarcted hearts; exercise time was about 14 min and maximal VO2 was 24.77 ± 5.00 mL·kg-1·min-1. These data show that hUCB therapy did not improve the cardiac function of infarcted animals or prevent cardiac remodeling.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Trasplante de Células Madre de Sangre del Cordón Umbilical / Infarto del Miocardio Límite: Animals / Humans Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Asunto de la revista: BIOLOGIA / MEDICINA Año: 2010 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Trasplante de Células Madre de Sangre del Cordón Umbilical / Infarto del Miocardio Límite: Animals / Humans Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Asunto de la revista: BIOLOGIA / MEDICINA Año: 2010 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil