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Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus L
Fiuza, Tatiana S; Silva, Paulo C; Paula, José R. de; Tresvenzol, Leonice M. F; Sabóia-Morais, Simone M. T.
Afiliación
  • Fiuza, Tatiana S; Institute of Biological Science. BR
  • Silva, Paulo C; School of Veterinary Medicine. Department of Animal Production. BR
  • Paula, José R. de; Federal University of Goiás. Faculty of Pharmacy. Goiânia. BR
  • Tresvenzol, Leonice M. F; Federal University of Goiás. Faculty of Pharmacy. Goiânia. BR
  • Sabóia-Morais, Simone M. T; Institute of Biological Science. BR
Biol. Res ; 42(4): 403-414, 2009. ilus
Article en En | LILACS | ID: lil-537100
Biblioteca responsable: BR1.1
ABSTRACT
This study evaluates the bioactivity of the crude ethanol extract and ethyl acetate, hexane and chloroform fractions obtained from Eugenia uniflora leaves using the hepatopancreas of Oreochromis niloticus L. as an experimental model. The ethanol extract and fractions were administered to the fish orally with their feed. Twenty-four hours later, the fish were sacrificed and their livers dissected, fixed in neutral formalin, embedded in paraffin and sectioned. Histological analyses were performed using Masson's trichrome and Haematoxylin-Eosin. Histochemical studies were performed using Feulgen, PAS (Periodic Acid Schiff) and PAS + salivary amylase and Sudan IV stain. The qualitative analysis of the material showed that the crude extract and the ethyl, chloroform and hexane fractions induced vasodilation, vascular congestion and toxicity due to the presence of eosinophilic granular cells, rodlet cells, some leukocytic infiltrate and rare focal necroses. The Nile tilapia proved to be a satisfactory model for screening plant products.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Extractos Vegetales / Cíclidos / Myrtaceae / Hepatopáncreas / Etanol Tipo de estudio: Qualitative_research Límite: Animals Idioma: En Revista: Biol. Res Asunto de la revista: BIOLOGIA Año: 2009 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Chile
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Extractos Vegetales / Cíclidos / Myrtaceae / Hepatopáncreas / Etanol Tipo de estudio: Qualitative_research Límite: Animals Idioma: En Revista: Biol. Res Asunto de la revista: BIOLOGIA Año: 2009 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Chile