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Experimental murine chromoblastomycosis obtained from Fonsecaea pedrosoi isolate cultured for a long periodt
Machado, A. P; Freymuller, E; Fischman, O.
Afiliación
  • Machado, A. P; Federal University of Mato Grosso. Department of Basic Health Sciences. Cuiabá. BR
  • Freymuller, E; Federal University of São Paulo. Department of Microbiology, Immunology and Parasitology. São Paulo. BR
  • Fischman, O; Federal University of São Paulo. Department of Microbiology, Immunology and Parasitology. São Paulo. BR
J. venom. anim. toxins incl. trop. dis ; J. venom. anim. toxins incl. trop. dis;15(4): 680-695, 2009. ilus
Article en En | LILACS | ID: lil-532753
Biblioteca responsable: BR33.1
ABSTRACT
The present study aimed to describe F. pedrosoi propagules capable of causing chronic murine disease. Several changes in F. pedrosoi hyphae were identified in fungal cells cultured for a long period. Optical microscopy found many rounded cells with double-rigid melanin-rich walls. Terminal and intercalary chlamydoconidia were also frequently observed. Analyses of images from transmission electron microscopy (TEM) revealed several cells with walls composed of at least three layers and an outer layer enriched with melanin. Two groups of twenty BALB/c mice were subcutaneously infected in their footpads with F. pedrosoi cells at an inoculum concentration of approximately 1 x 10(4) cells/mL. In one group, long-term cultured F. pedrosoi cells were inoculated in one footpad, whereas in the other group, both footpads were infected. Active lesions were observed up to seven months post-infection, particularly in mice inoculated at two sites. After this period, animals were killed. Histological sections revealed characteristics bearing a strong resemblance to the human form of the disease such as tissue hyperplasia, granulomas with microabscesses and sclerotic cells. Based on this study, we identified fungal cells from old cultures capable of provoking chronic chromoblastomycosis under experimental conditions, especially when more than one site is infected.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Cromoblastomicosis / Murinae / Hongos / Ratones Endogámicos BALB C Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Asunto de la revista: TOXICOLOGIA Año: 2009 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Cromoblastomicosis / Murinae / Hongos / Ratones Endogámicos BALB C Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J. venom. anim. toxins incl. trop. dis Asunto de la revista: TOXICOLOGIA Año: 2009 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil