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RNAi-mediated silencing of the Bmi-1 gene causes growth inhibition and enhances doxorubicin-induced apoptosis in MCF-7 cells
Wu, Xiang-mei; Liu, Xing; Bu, You-quan; Sengupta, Joyeeta; Cui, Hong-juan; Yi, Fa-ping; Liu, Tao; Yuan, Chen-fu; Shi, Yan-yan; Song, Fang-zhou.
Afiliación
  • Wu, Xiang-mei; Chongqing Medical University. Department of Biochemistry and Molecular Biology. Chongqing. CN
  • Liu, Xing; Chongqing Medical University. Chongqing Children's Hospital. Department of Pediatric Urology. Chongqing. CN
  • Bu, You-quan; Chongqing Medical University. Department of Biochemistry and Molecular Biology. Chongqing. CN
  • Sengupta, Joyeeta; Chongqing Medical University. Department of Biochemistry and Molecular Biology. Chongqing. CN
  • Cui, Hong-juan; Medical University of Ohio. Department of Biochemistry and Cancer Biology. Ohio. US
  • Yi, Fa-ping; Chongqing Medical University. Department of Biochemistry and Molecular Biology. Chongqing. CN
  • Liu, Tao; Chongqing Medical University. Department of Biochemistry and Molecular Biology. Chongqing. CN
  • Yuan, Chen-fu; Chongqing Medical University. Department of Biochemistry and Molecular Biology. Chongqing. CN
  • Shi, Yan-yan; Chongqing Medical University. Department of Biochemistry and Molecular Biology. Chongqing. CN
  • Song, Fang-zhou; Chongqing Medical University. Department of Biochemistry and Molecular Biology. Chongqing. CN
Genet. mol. biol ; Genet. mol. biol;32(4): 697-703, 2009. ilus, graf, tab
Article en En | LILACS | ID: lil-531805
Biblioteca responsable: BR1.1
ABSTRACT
The oncogene Bmi-1 is a member of the Polycomb group gene family. Its expression is found to be greatly increased in a number of malignant tumors including breast cancer. This could suggest Bmi-1 as a potent therapeutic target. In this study, RNAi was introduced to down-regulate the expression of Bmi-1 in a highly malignant breast adenocarcinoma cell line, MCF-7. A thorough study of the biological behavior and chemosensitivity changes of the MCF-7 cells was carried out in context to the therapeutic potential of Bmi-1. The results obtained indicated that siRNA targeting of Bmi-1 could lead to an efficient and specific inhibition of endogenous Bmi-1 activity. The mRNA and protein expression of Bmi-1 were determined by RT-PCR and Western blot, respectively. Furthermore, silencing of Bmi-1 resulted in a drastic inhibition of the growth of MCF-7 cells as well as G1/S phase transition. The number of target cells was found to increase in phase G0/G1 and decrease in the S phase, but no increase in the basal level of apoptosis was noticed. On the other hand, a reduction in the expression of cyclin D1 and an increase in the expression of p21 were also noticed. Silencing of Bmi-1 made the MCF-7 cells more sensitive to the chemotherapeutic agent doxorubicin and induced a significantly higher percentage of apoptotic cells. Here, we report on a study regarding the RNAi-mediated silencing of the Bmi-1 gene in breast cancer.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Tipo de estudio: Etiology_studies Idioma: En Revista: Genet. mol. biol Asunto de la revista: GENETICA Año: 2009 Tipo del documento: Article País de afiliación: China / Estados Unidos Pais de publicación: Brasil
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Tipo de estudio: Etiology_studies Idioma: En Revista: Genet. mol. biol Asunto de la revista: GENETICA Año: 2009 Tipo del documento: Article País de afiliación: China / Estados Unidos Pais de publicación: Brasil