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Analysis of Enterococcus faecalis in samples from Turkish patients with primary endodontic infections and failed endodontic treatment by real-time PCR SYBR green method
Ozbek, Selcuk M; Ozbek, Ahmet; Erdorgan, Aziz S.
Afiliación
  • Ozbek, Selcuk M; Ataturk University. Dental School. Department of Endodontics. Erzurum. TR
  • Ozbek, Ahmet; Ataturk University. Medical Faculty. Department of Microbiology and Clinical Microbiology. Erzurum. TR
  • Erdorgan, Aziz S; Ataturk University. Dental School. Department of Endodontics. Erzurum. TR
J. appl. oral sci ; 17(5): 370-374, Sept.-Oct. 2009.
Article en En | LILACS | ID: lil-531381
Biblioteca responsable: BR1.1
ABSTRACT

OBJECTIVE:

The aims of this study were to investigate the presence of Enterococcus faecalis in primary endodontic infections and failed endodontic treatments using real-time PCR and to determine the statistical importance of the presence of E. faecalis in a Turkish population with endodontic infections. MATERIAL AND

METHODS:

E. faecalis was investigated from 79 microbial samples collected from patients who were treated at the Endodontic Clinic of the Dental School of Atatürk University (Erzurum, Turkey). Microbial samples were taken from 43 patients (Group 1) with failed endodontic treatments and 36 patients (Group 2) with chronic apical periodontitis (primary endodontic infections). DNA was extracted from the samples by using a QIAamp® DNA mini-kit and analyzed with real-time PCR SYBR Green.

RESULTS:

E. faecalis was detected in 41 out of 79 patients, suggesting that it exists in not less than 61 percent of all endodontic infections when the proportion test (z= -1.645, Real-time PCR SYBR Green allowed for the detection of E. faecalis in 32 out of 43 (74.4 percent) in Group 1, and in 9 out of 36 (25 percent) in Group 2.

CONCLUSIONS:

These results suggest that E. faecalis is a frequent isolate for endodontic infections in Turkish patients, and is more often associated with failed endodontic treatments than primary endodontic infections.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Tratamiento del Conducto Radicular / Reacción en Cadena de la Polimerasa / Infecciones por Bacterias Grampositivas / Enterococcus faecalis / Enfermedades de la Pulpa Dental Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J. appl. oral sci Asunto de la revista: ODONTOLOGIA Año: 2009 Tipo del documento: Article / Project document País de afiliación: Turquía Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Tratamiento del Conducto Radicular / Reacción en Cadena de la Polimerasa / Infecciones por Bacterias Grampositivas / Enterococcus faecalis / Enfermedades de la Pulpa Dental Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J. appl. oral sci Asunto de la revista: ODONTOLOGIA Año: 2009 Tipo del documento: Article / Project document País de afiliación: Turquía Pais de publicación: Brasil