Prostaglandin A1 inhibits the replication of Sindbis virus in monkey kidney and mosquito cells
Rev. Ciênc. Méd. Biol. (Impr.)
; 7(2): 169-174, maio-ago. 2008. ilus
Article
en En
| LILACS, BBO
| ID: lil-530648
Biblioteca responsable:
BR337.1
ABSTRACT
The present study reports the effect of prostaglandin A1 (PGA1) on the replication of Sindbis virus in monkey kidney and mosquito cells. In PGA1 treated cells we observed a severe reduction of virus yield. In both cells lines the highest nontoxic concentration of PGA1 (10 ìg/mL) decreased virus replication, dose dependently, by more than 90 por cento. SDS-PAGE analysis of [35S] methionine labeled proteins showed that viral proteins (E1/E2 and C) were normally synthesized in PGA1 treated Vero cells, and induction of stress proteins (HSP70 and HSP90 ) was detected in uninfected and infected cells. In Vero cells the inhibition of virus replication was accompanied by a decrease in [3H] glucosamine incorporation into the virus glycoproteins.
Texto completo:
1
Colección:
01-internacional
Base de datos:
BBO
/
LILACS
Asunto principal:
Prostaglandinas A
/
Virus Sindbis
/
Células Vero
Idioma:
En
Revista:
Rev. Ciênc. Méd. Biol. (Impr.)
Asunto de la revista:
BIOLOGIA
/
MEDICINA
Año:
2008
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil