Your browser doesn't support javascript.
loading
LEPR p. Q223R, Beta 3-AR p. W64R and LEPc. -2548G>A gene variants in obese Brazilian subjects
Duarte, S. F. P; Francischetti, E. A; Genelhu, V. A; Cabello, P. H; Pimentel, M. M. G.
Afiliación
  • Duarte, S. F. P; Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Biologia Celular e Genética. Rio de Janeiro. BR
  • Francischetti, E. A; Universidade do Estado do Rio de Janeiro. Escola de Medicina. Laboratório de Fisiopatologia Clínica e Experimental. Rio de Janeiro. BR
  • Genelhu, V. A; Universidade do Estado do Rio de Janeiro. Escola de Medicina. Laboratório de Fisiopatologia Clínica e Experimental. Rio de Janeiro. BR
  • Cabello, P. H; Ministério da Saúde. Instituto Oswaldo Cruz. Rio de Janeiro. BR
  • Pimentel, M. M. G; Universidade do Estado do Rio de Janeiro. Instituto de Biologia Roberto Alcântara Gomes. Departamento de Biologia Celular e Genética. Rio de Janeiro. BR
Genet. mol. res. (Online) ; Genet. mol. res. (Online);6(4): 1035-1043, 2007. tab
Article en En | LILACS | ID: lil-520046
Biblioteca responsable: BR26.1
ABSTRACT
Obesity is due to the combined effects of genes, environment, lifestyle, and the interactions of these factors. The adrenergic receptor ¦Â3 (¦Â3-AR), leptin (LEP) and leptin receptor (LEPR) genes have been intensively evaluated in the search of variants that could be related to obesity and its cardiometabolic complications. The results of most of these studies have been controversial. In the present study, we investigated the relationship of the ¦Â3-AR p.W64R, LEP c.-2548G>A and LEPR p.Q223R gene variants with body mass index (BMI), in Brazilian subjects of different genetic backgrounds and ethnic origins. Two hundred obese patients (60 males, 140 females, BMI ¡Ý 30 kg/m2) were screened and compared to 150 lean healthy subjects (63 males, 87 females, BMI ¡Ü 24 kg/m2). Genomic DNA was extracted and amplified by polymerase chain reaction. Polymerase chain reaction products were digested with specific restriction enzymes and separated by electrophoresis. There was no significant difference in the genotype frequency of the ¦Â3-AR p.W64R and the LEP c.-2548G>A polymorphisms, between lean and obese subjects. However, the genotype and allele frequencies of the LEPR p.Q223R variant were significantly different between the normal weight and obese groups. Haplotype analysis has shown an association between the G/G allelic combination of c.-2548G>A LEP and c.668A>G LEPR, in obese subjects. Our results suggest that genetic variability in the leptin receptor is associated with body weight regulation, the LEPR p.Q223R variant being related to BMI increase. The haplotype combination of LEP c.-2548G>A and LEPR p.Q223R variants was related to a 58% increase in obesity risk.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Variación Genética / Obesidad Tipo de estudio: Observational_studies / Risk_factors_studies Límite: Adolescent / Adult / Female / Humans / Male País/Región como asunto: America do sul / Brasil Idioma: En Revista: Genet. mol. res. (Online) Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2007 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Variación Genética / Obesidad Tipo de estudio: Observational_studies / Risk_factors_studies Límite: Adolescent / Adult / Female / Humans / Male País/Región como asunto: America do sul / Brasil Idioma: En Revista: Genet. mol. res. (Online) Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2007 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil