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Cross-amplification tests of ungulate primers in the endangered Neotropical pampas deer (Ozotoceros bezoarticus)
Cosse, M; González, S; Maldonado, J. E.
Afiliación
  • Cosse, M; Unidad Asociada de Facultad de Ciencias. IIBCE. Departamento de Genética. Montevideo. UY
  • González, S; Unidad Asociada de Facultad de Ciencias. IIBCE. Departamento de Genética. Montevideo. UY
  • Maldonado, J. E; Smithsonian Institution. Center for Conservation and Evolutionary Genetics. Washington. US
Genet. mol. res. (Online) ; Genet. mol. res. (Online);6(4): 1118-1122, 2007. tab
Article en En | LILACS | ID: lil-520037
Biblioteca responsable: BR26.1
ABSTRACT
In cross-species amplification tests of 15 ungulate primers in pampas deer, five were retained to form a small panel of highly polymorphic loci that could be used to efficiently screen populations of this endangered species. The polymerase chain reactions were performed incorporating the universal fluorescent labeled M13 (-21) primer. In 69 pampas deer, average allelic diversity was 15, expected heterozygosity was 0.869 and the mean polymorphic information content value was 0.847. Paternity exclusion probabilities over loci were NE-1P = 0.01336 and NE-2P = 0.00135, and combined non-exclusion probability of identity was P(ID) = 3 × 10-8.
Asunto(s)
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Ciervos / Desequilibrio de Ligamiento / Repeticiones de Microsatélite Límite: Animals Idioma: En Revista: Genet. mol. res. (Online) Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2007 Tipo del documento: Article País de afiliación: Estados Unidos / Uruguay Pais de publicación: Brasil
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Ciervos / Desequilibrio de Ligamiento / Repeticiones de Microsatélite Límite: Animals Idioma: En Revista: Genet. mol. res. (Online) Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2007 Tipo del documento: Article País de afiliación: Estados Unidos / Uruguay Pais de publicación: Brasil