Cross-amplification tests of ungulate primers in the endangered Neotropical pampas deer (Ozotoceros bezoarticus)
Genet. mol. res. (Online)
; Genet. mol. res. (Online);6(4): 1118-1122, 2007. tab
Article
en En
| LILACS
| ID: lil-520037
Biblioteca responsable:
BR26.1
ABSTRACT
In cross-species amplification tests of 15 ungulate primers in pampas deer, five were retained to form a small panel of highly polymorphic loci that could be used to efficiently screen populations of this endangered species. The polymerase chain reactions were performed incorporating the universal fluorescent labeled M13 (-21) primer. In 69 pampas deer, average allelic diversity was 15, expected heterozygosity was 0.869 and the mean polymorphic information content value was 0.847. Paternity exclusion probabilities over loci were NE-1P = 0.01336 and NE-2P = 0.00135, and combined non-exclusion probability of identity was P(ID) = 3 × 10-8.
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Ciervos
/
Desequilibrio de Ligamiento
/
Repeticiones de Microsatélite
Límite:
Animals
Idioma:
En
Revista:
Genet. mol. res. (Online)
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos
/
Uruguay
Pais de publicación:
Brasil