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Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires
Schreier, Stefan; Triampo, Wannapong; Doungchawee, Galayanee; Triampo, Darapond; Chadsuthi, Sudarat.
Afiliación
  • Schreier, Stefan; University of Applied Science Munich. Faculty of engineering physics. Department of Bioengineering. DE
  • Triampo, Wannapong; Mahidol University. Faculty of Science. Department of Physics. Group of Biological and Environmental Physics. Bangkok. TH
  • Doungchawee, Galayanee; Mahidol University. Faculty of Science. Department of Pathobiology. Bangkok. TH
  • Triampo, Darapond; Mahidol University. Faculty of Science. Department of Physics. Group of Biological and Environmental Physics. Bangkok. TH
  • Chadsuthi, Sudarat; Mahidol University. Faculty of Science. Department of Physics. Group of Biological and Environmental Physics. Bangkok. TH
Biol. Res ; 42(1): 5-12, 2009. ilus
Article en En | LILACS | ID: lil-519079
Biblioteca responsable: BR1.1
ABSTRACT
Leptospirosis caused by Leptospira interrogans is the most widespread zoonosis and a major public health problem worldwide. Based on light-scattering and absorption, quantification of leptospires using UV-VIS spectroscopy was used as an indirect counting technique by measuring the optical density and comparing this to automated direct counting using a counting chamber in combination with imaging and analyzing software. Two serovars, Bangkok and Copenhagenii, from log-phase growth were used for the establishment of standard curves. They were found to be linear and slightly different in gradient for each serovar. The ease, rapidity, and reliability of these two adapted and optimized counting techniques may provide a useful alternative enumeration technique for leptospirosis research.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Leptospira interrogans / Leptospirosis Límite: Humans Idioma: En Revista: Biol. Res Asunto de la revista: BIOLOGIA Año: 2009 Tipo del documento: Article País de afiliación: Alemania / Tailandia Pais de publicación: Chile
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Leptospira interrogans / Leptospirosis Límite: Humans Idioma: En Revista: Biol. Res Asunto de la revista: BIOLOGIA Año: 2009 Tipo del documento: Article País de afiliación: Alemania / Tailandia Pais de publicación: Chile