Your browser doesn't support javascript.
loading
Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection
Martins-Souza, R. L; Pereira, C. A. J; Martins Filho, O. A; Coelho, P. M. Z; Corrêa Júnior, A; Negrão-Corrêa, D.
Afiliación
  • Martins-Souza, R. L; Fiocruz. Centro de Pesquisa René-Rachou. Departamento de Parasitologia. Belo Horizonte. BR
  • Pereira, C. A. J; Fiocruz. Centro de Pesquisa René-Rachou. Departamento de Parasitologia. Belo Horizonte. BR
  • Martins Filho, O. A; Fiocruz. Centro de Pesquisa René-Rachou. Belo Horizonte. BR
  • Coelho, P. M. Z; Fiocruz. Centro de Pesquisa René-Rachou. Belo Horizonte. BR
  • Corrêa Júnior, A; UFMG. ICB. Departamento de Microbiologia. Belo Horizonte. BR
  • Negrão-Corrêa, D; Fiocruz. Centro de Pesquisa René-Rachou. Departamento de Parasitologia. Belo Horizonte. BR
Mem. Inst. Oswaldo Cruz ; 101(supl.1): 185-192, Oct. 2006. graf, ilus
Article en En | LILACS | ID: lil-441245
Biblioteca responsable: BR1.1
ABSTRACT
Lectins/carbohydrate binding can be involved in the Schistosoma mansoni recognition and activation of the Biomphalaria hemocytes. Therefore, expression of lectin ligands on Biomphalaria hemocytes would be associated with snail resistance against S. mansoni infection. To test this hypothesis, circulating hemocytes were isolated from B. glabrata BH (snail strain highy susceptible to S. mansoni), B. tenagophila Cabo Frio (moderate susceptibility), and B. tenagophila Taim (completely resistant strains), labelled with FITC conjugated lectins (ConA, PNA, SBA, and WGA) and analyzed under fluorescence microscopy. The results demonstrated that although lectin-labelled hemocytes were detected in hemolymph of all snail species tested, circulating hemocytes from both strains of B. tenagophila showed a larger number of lectin-labelled cells than B. glabrata. Moreover, most of circulating hemocytes of B. tenagophila were intensively labelled by lectins PNA-FITC and WGA-FITC, while in B. glabrata small hemocytes were labeled mainly by ConA. Upon S. mansoni infection, lectin-labelled hemocytes almost disappeared from the hemolymph of Taim and accumulated in B. glabrata BH. The role of lectins/carbohydrate binding in resistance of B. tengophila infection to S. mansoni is still not fully understood, but the data suggest that there may be a correlation to its presence with susceptibility or resistance to the parasite.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Schistosoma mansoni / Biomphalaria / Hemocitos / Lectinas Límite: Animals Idioma: En Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: MEDICINA TROPICAL / PARASITOLOGIA Año: 2006 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Schistosoma mansoni / Biomphalaria / Hemocitos / Lectinas Límite: Animals Idioma: En Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: MEDICINA TROPICAL / PARASITOLOGIA Año: 2006 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil