Occupational genotoxicity risk evaluation through the comet assay and the micronucleus test
Genet. mol. res. (Online)
; 2(4): 410-4177, Dec. 2003.
Article
en En
| LILACS
| ID: lil-417589
Biblioteca responsable:
BR1.1
ABSTRACT
The micronucleus (MN) test and the alkaline single cell gel or comet assay were applied to exfoliated cells of the buccal mucous in order to evaluate the genotoxic risk associated with occupational exposure of 10 storage battery renovation workers, and 10 car painters, with age matched controls, in Pelotas, RS, in southern Brazil. In the MN test, 2000 exfoliated buccal cells were analyzed for each individual, while 100 cells were examined in the comet assay. In the comet test, both comet tail length and a damage index were calculated. Highly significant effects of occupational exposure were found with both the MN test and the comet assay (P<0.001). The comet assay was found to be rapid, of simple visualization, and it is a sensitive technique for measuring and analyzing DNA damage in human cells
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Pintura
/
Daño del ADN
/
Exposición Profesional
/
Contaminantes Ocupacionales del Aire
/
Plomo
Tipo de estudio:
Etiology_studies
/
Observational_studies
/
Risk_factors_studies
Límite:
Adult
/
Humans
/
Male
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Genet. mol. res. (Online)
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil