Microsatellites in Portuguese autochthonous horse breeds: usefulness for parentage testing

Lu¡s, C; Cothran, E; Gus and Oom, M. M

Lu¡s, C; Cothran, E; Gus and Oom, M. M.

Afiliación

Lu¡s, C; Universidade de Lisboa. Faculdade de Ciências. Lisboa. PT
Cothran, E; University of Kentucky. Equine Blood Typing Research Laboratory. Lexington. US
Gus and Oom, M. M; Universidade de Lisboa. Faculdade de Ciências. Lisboa. PT

Genet. mol. biol ; 25(2): 131-134, Jun. 2002. tab

Article en En | LILACS | ID: lil-335781

Biblioteca responsable: BR26.1

ABSTRACT

ABSTRACT

The use of DNA technology for parentage testing is increasing every day. Most laboratories have improved their exclusion probabilities (PE) by the addition of DNA microsatellite loci to standard blood-typing results. The efficacy of each locus depends on the number of alleles detected and their frequencies in the breeds tested. Here we analyzed the usefulness of six microsatellites for routine parentage testing in three Portuguese autochthonous horse breeds Lusitano, Sorraia and Garrano. The DNA loci analyzed - ASB2, HMS3, HMS7, HTG4, HTG10, and VHL20 - were chosen based upon the polymorphism detected in other breeds with Iberian horse influence. The estimated probability of exclusion of wrongly named parents (PE) was high, with values ranging from 88.5 per cent to 99.6 per cent

Asunto(s)

Animales; ADN; Caballos; Reacción en Cadena de la Polimerasa; Repeticiones de Microsatélite/genética; Marcadores Genéticos; Paternidad

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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: ADN / Reacción en Cadena de la Polimerasa / Repeticiones de Microsatélite / Caballos Límite: Animals Idioma: En Revista: Genet. mol. biol Asunto de la revista: GENETICA Año: 2002 Tipo del documento: Article País de afiliación: Portugal / Estados Unidos Pais de publicación: Brasil

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