Detection of Neisseria meningitidis in cerebrospinal fluid samples from suspicious cases of meningococcal meningitis using polymerase chain reaction and counterimmunoelectrophoresis
Rev. argent. microbiol
; Rev. argent. microbiol;32(2): 97-103, abr.-jun. 2000.
Article
en En
| LILACS
| ID: lil-332530
Biblioteca responsable:
BR1.1
ABSTRACT
Rapid diagnosis of meningococcal disease followed by an early treatment is essential. However, blood or cerebrospinal fluid cultures may not be successful because antibiotic treatment is often started before proper specimens are collected and because bacteria may die during transportation to the laboratory. Improvements in antibiotic therapy for specific microorganisms will require the use of more than one method for immunodiagnosis. In this study a collection of cerebrospinal fluid samples from Brazilian patients was analyzed. Gram stains, culture, counterimmunoelectrophoresis and clinical evaluations for meningococcal diseases were available. The sensitivity of nested PCR (nPCR) was 73 for cerebrospinal fluid of clinically suspected cases, whereas both sensitivity and specificity were 100 when subtypes of Brazilian epidemic strains (P1.7, P1.9 and P1.15) isolated from the samples were used.
Buscar en Google
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Meningitis Meningocócica
/
Neisseria meningitidis
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Rev. argent. microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2000
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Argentina