Phenotyping and genotyping methods applied to investigate the relatedness of Brazilian isolates of Enterobacter cloacae
Braz. j. med. biol. res
; 32(9): 1077-81, Sept. 1999.
Article
en En
| LILACS
| ID: lil-241600
Biblioteca responsable:
BR1.1
RESUMO
In order to evaluate the resolving power of several typing methods to identify relatedness among Brazilian strains of Enterobacter cloacae, we selected twenty isolates from different patients on three wards of a University Hospital (Orthopedics, Nephrology, and Hematology). Traditional phenotyping methods applied to isolates included biotyping, antibiotic sensitivity, phage-typing, and O-serotyping. Plasmid profile analysis, ribotyping, and macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) were used as genotyping methods. Sero- and phage-typing were not useful since the majority of isolates could not be subtyped by these methods. Biotyping, antibiogram and plasmid profile permitted us to classify the samples into different groups depending on the method used, and consequently were not reliable. Ribotyping and PFGE were significantly correlated with the clinical epidemiological analysis. PFGE did not type strains containing nonspecific DNase. Ribotyping was the most discriminative method for typing Brazilian isolates of E. cloacae
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Técnicas de Tipificación Bacteriana
/
Enterobacter cloacae
Límite:
Humans
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Braz. j. med. biol. res
Asunto de la revista:
BIOLOGIA
/
MEDICINA
Año:
1999
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil