Effect of prostaglandin A1 in the induction of stress proteins in Aedes albopictus cells
Rev. bras. pesqui. méd. biol
; Braz. j. med. biol. res;31(4): 499-503, Apr. 1998. ilus
Article
en En
| LILACS
| ID: lil-212412
Biblioteca responsable:
BR1.1
ABSTRACT
Prostaglandin are natural fatty acid derivatives with diverse physiological effects, including immune function and the control of cell growth. While the action of prostaglandins in the induction of stress proteins in vertebrate cells in well documented, their functions in invertebrate cells have been poorly investigated. The purpose of the present study was to investigate the effect of prostaglandin A1 (PGA1; 0.25, 1.25 and 12.5 mug/ml) on protein synthesis during the growth of Aedes albopictus cells. We found that PGA1 stimulates the synthesis of several polypeptides with molecular masses of 87,80,70,57,29,27 and 23 kDa in Aedes albopictus cells. When the protein induced by PGA1 and those induced by heat treatment were compared by polyacrylamide gel electrophoresis, PGA1 was found to induce the stress proteins. The HSP70 family and the low-molecular weight polypeptides (29 and 27 kDa, respectively) were induced by PGA1 in the lag phase. We lso observed that PGA1 is able to induce a 23-kDa polypeptide independently of the growth phase of the cell.
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Prostaglandinas A
/
Aedes
/
Proteínas de Choque Térmico
Límite:
Animals
Idioma:
En
Revista:
Braz. j. med. biol. res
/
Rev. bras. pesqui. méd. biol
Asunto de la revista:
BIOLOGIA
/
MEDICINA
Año:
1998
Tipo del documento:
Article
Pais de publicación:
Brasil