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Uropathogenic bacteria and deductive genomics towards antimicrobial resistance, virulence, and potential drug targets / Bacterias uropatógenas y genómica deductiva hacia la resistencia, virulencia y posibles objetivos farmacológicos a los antimicrobianos
Amin, Aaima; Noureen, Ramisha; Iftikhar, Ayesha; Hussain, Annam; Alonazi, Wadi B; Zeeshan Raza, Hafiz Muhammad; Ferheen, Ifra; Ibrahim, Muhammad.
Afiliación
  • Amin, Aaima; COMSATS University Islamabad. Department of Biosciences. Sahiwal. Pakistan
  • Noureen, Ramisha; Fatima Jinnah Medical University. Medical Department. Lahore. Pakistan
  • Iftikhar, Ayesha; Govt Khawaja Muhammad Safdar Medical Collage. Medical Department. Sialkot. Pakistan
  • Hussain, Annam; COMSATS University Islamabad. Department of Biosciences. Sahiwal. Pakistan
  • Alonazi, Wadi B; College of Business Administration, King Saud University. Health Administration Department. Riyadh. Saudi Arabia
  • Zeeshan Raza, Hafiz Muhammad; COMSATS University Islamabad. Department of Biosciences. Sahiwal. Pakistan
  • Ferheen, Ifra; University of Camerino. School of Biosciences and Veterinary Medicine. Laboratory of Genetics of Microorganisms. Camerino. Italy
  • Ibrahim, Muhammad; COMSATS University Islamabad. Department of Biosciences. Sahiwal. Pakistan
Int. microbiol ; 27(1): 325-335, Feb. 2024. mapas
Article en En | IBECS | ID: ibc-230264
Biblioteca responsable: ES1.1
Ubicación: ES15.1 - BNCS
ABSTRACT
Urinary tract infections (UTIs) are among the most prevalent bacterial infections affecting people in inpatient and outpatient settings. The current study aimed to sequence the genome of uropathogenic Escherichia coli strain CUI-B1 resourced from a woman having uncomplicated cystitis and pyelonephritis. Followed by deductive genomics towards potential drug targets using E. coli strain CUI-B1, strain O25b: H4-ST131, Proteus mirabilis strain HI4320, Klebsiella pneumoniae strain 1721, and Staphylococcus saprophyticus strain ATCC 15305 uropathogenic strains. Comparative genome analysis revealed that genes related to the survival of E. coli, P. mirabilis, K. pneumoniae, and S. saprophyticus, such as genes of metal-requiring proteins, defense-associated genes, and genes associated with general physiology, were found to be highly conserved in the genomes including strain CUI-B1. However, the genes responsible for virulence and drug resistance, mainly those that are involved in bacterial secretion, fimbriae, adherence, and colonization, were found in various genomic regions and varied from one species to another or within the same species. Based on the genome sequence, virulence, and antimicrobial-resistant gene dataset, the subtractive proteomics approach revealed 22 proteins mapped to the pathogen’s unique pathways and among them, entB, clbH, chuV, and ybtS were supposed to be potential drug targets and the single drug could be utilized for all above-mentioned strains. These results may provide the foundation for the optimal target for future discovery of drugs for E. coli-, P. mirabilis-, K. pneumoniae-, and S. saprophyticus-based infections and could be investigated further to employ in personalized drug development.(AU)
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Colección: 06-national / ES Base de datos: IBECS Asunto principal: Infecciones Urinarias / Virulencia / Resistencia a Medicamentos / Factores de Virulencia / Escherichia coli / Antibacterianos Límite: Humans Idioma: En Revista: Int. microbiol Año: 2024 Tipo del documento: Article
Buscar en Google
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Colección: 06-national / ES Base de datos: IBECS Asunto principal: Infecciones Urinarias / Virulencia / Resistencia a Medicamentos / Factores de Virulencia / Escherichia coli / Antibacterianos Límite: Humans Idioma: En Revista: Int. microbiol Año: 2024 Tipo del documento: Article