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JHDM1D-AS1-driven inhibition of miR-940 releases ARTN expression to induce breast carcinogenesis
Zuo, Yonggang; Ma, Mingde; Wen, Yuqing; Chang, Liang; Qu, Changping.
Afiliación
  • Zuo, Yonggang; Henan University. Huaihe Hospital. Department of Breast and Thyroid Surgery. Kaifeng. People’s Republic of China
  • Ma, Mingde; Henan University. Huaihe Hospital. Department of Breast and Thyroid Surgery. Kaifeng. People’s Republic of China
  • Wen, Yuqing; Henan University. Huaihe Hospital. Department of Breast and Thyroid Surgery. Kaifeng. People’s Republic of China
  • Chang, Liang; Henan University. Huaihe Hospital. Department of Breast and Thyroid Surgery. Kaifeng. People’s Republic of China
  • Qu, Changping; Henan University. Huaihe Hospital. Department of Obstetrics and Gynecology. Kaifeng. People’s Republic of China
Clin. transl. oncol. (Print) ; 25(7): 2191-2203, jul. 2023. ilus, graf
Article en En | IBECS | ID: ibc-222388
Biblioteca responsable: ES1.1
Ubicación: ES15.1 - BNCS
ABSTRACT
Introduction As ceRNA network of long non-coding RNA (lncRNA)–microRNA (miR)–messenger RNAs (mRNA) can be predicted on the basis of bioinformatics tools, we are now one step closer to deeper understanding carcinogenic mechanisms. In this study, we clarified the mechanistic understanding of JHDM1D-AS1-miR-940-ARTN ceRNA network in the development of breast cancer (BC). Materials and Methods The lncRNA–miRNA–mRNA interaction of interest was predicted by in silico analysis and identified by conducting RNA immunoprecipitation, RNA pull-down and luciferase assays. The expression patterns of JHDM1D-AS1, miR-940 and ARTN in BC cells were altered by lentivirus infection and plasmid transfection for functional assays on the biological properties of BC cells. Finally, the tumorigenic and metastatic abilities of BC cells were assessed in vivo. Results JHDM1D-AS1 was highly expressed, while miR-940 was poorly expressed in BC tissues and cells. JHDM1D-AS1 could competitively bind to miR-940, whereby promoting the malignant behaviors of BC cells. Furthermore, ARTN was identified as a target gene of miR-940. Through targeting ARTN, miR-940 exerted a tumor-suppressive role. In vivo experiments further confirmed that JHDM1D-AS1 enhanced the tumorigenesis and metastasis through up-regulation of ARTN. Conclusions Taken together, our study demonstrated the involvement of ceRNA network JHDM1D-AS1-miR-940-ARTN in the progression of BC, which highlighted promising therapeutic targets for BC treatment (AU)
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Colección: 06-national / ES Base de datos: IBECS Asunto principal: Neoplasias de la Mama / MicroARNs / ARN Largo no Codificante / Carcinogénesis Límite: Humans Idioma: En Revista: Clin. transl. oncol. (Print) Año: 2023 Tipo del documento: Article
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Colección: 06-national / ES Base de datos: IBECS Asunto principal: Neoplasias de la Mama / MicroARNs / ARN Largo no Codificante / Carcinogénesis Límite: Humans Idioma: En Revista: Clin. transl. oncol. (Print) Año: 2023 Tipo del documento: Article