Analysis of bacterial communities of infected primary teeth in a Mexican population
Med. oral patol. oral cir. bucal (Internet)
; 25(5): e668-e674, sept. 2020. tab, graf
Article
en En
| IBECS
| ID: ibc-196523
Biblioteca responsable:
ES1.1
Ubicación: BNCS
ABSTRACT
BACKGROUND: The objective of this study was to describe the bacterial communities associated with pediatric patients with endodontic infections of temporal teeth by targeting the 16S rRNA gene using pyrosequencing. MATERIAL AND METHODS: Microbiological samples were obtained from the lower primary molars of thirteen 13 pediatric patients with dental infections. An aspiration method for microbiological sampling was used. The identification of microbiota employing the pyrosequencing method by targeting the 16S gene was performed. RESULTS: Ribosomal 16S RNA gene sequences were amplified, obtaining a total of 16,182 sequences from 13 primary infected molars (13 different individuals) by pyrosequencing. Bacteroidetes phyla (35.15%) were the most abundant followed by Firmicutes (33.3%) and Fusobacteria (10.05%); the presence of specific pathogenic bacteria was determined as well. CONCLUSIONS: The infected root canal of primary teeth contains a high diversity of anaerobic bacteria, and Bacteroidetes, Firmicutes, and Fusobacteria phyla were the most abundant; Prevotella and Streptococcus genera were the most prevalent
RESUMEN
No disponible
Texto completo:
1
Colección:
06-national
/
ES
Base de datos:
IBECS
Asunto principal:
Bacterias
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Diente Primario
/
Cavidad Pulpar
Límite:
Child
/
Child, preschool
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Female
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Humans
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Male
País/Región como asunto:
Mexico
Idioma:
En
Revista:
Med. oral patol. oral cir. bucal (Internet)
Año:
2020
Tipo del documento:
Article