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GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes
Guerra, LN; Suarez, C; Soto, D; Schiappacasse, A; Sapochnik, D; Sacca, P; Piwien-Pilipuk, G; Peral, B; Calvo, JC.
Afiliación
  • Guerra, LN; Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Buenos Aires. Argentina
  • Suarez, C; Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Computación. Buenos Aires. Argentina
  • Soto, D; Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Buenos Aires. Argentina
  • Schiappacasse, A; Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Buenos Aires. Argentina
  • Sapochnik, D; Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Buenos Aires. Argentina
  • Sacca, P; Laboratorio de Proteoglicanos y Matriz Extracelular. Buenos Aires. Argentina
  • Piwien-Pilipuk, G; Instituto de Biología y Medicina Experimental (IByME). Laboratorio de Arquitectura Nuclear. Buenos Aires. Argentina
  • Peral, B; Consejo Superior de Investigaciones Científicas (CSIC). Instituto de Investigaciones Biomédicas, Alberto Sols. Madrid. Spain
  • Calvo, JC; Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Buenos Aires. Argentina
Clin. transl. oncol. (Print) ; 17(7): 511-520, jul. 2015. ilus
Article en En | IBECS | ID: ibc-138447
Biblioteca responsable: ES1.1
Ubicación: BNCS
ABSTRACT
Introduction. In the mammary gland, the involution that occurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal–epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et al., Clin Transl Oncol 15:124–131, 2013). Here, we evaluated the effects of conditioned medium of breast epithelial mammary cells on stromal cells. Materials and methods. Conditioned medium from normal murine mammary gland cell line (NMuMG) and conditioned medium proteins were obtained. Then, they were evaluated on modulation of adipocyte differentiation, using 3T3-L1 cell line. Results. We described, for the first time, that breast epithelial mammary cells could produce the enzyme galactose 3-O-sulfotransferase 2 (GAL3ST2). Importantly, GAL3ST2 is present in NMMuMG and two human breast cancer cell lines, and it is more strongly expressed in more metastatic tumors. When 3T3-L1 preadipocyte differentiation was triggered in the presence of conditioned medium from NMuMG or GAL3ST2, triglyceride accumulation was decreased by 40 % and C/EBPβ expression by 80 % in adipocytes. In addition, the expression of FABP4 (aP2), another marker of adipocyte differentiation, was inhibited by 40 % in GAL3ST2-treated cells. Conclusions. Taken together, these results suggest that GAL3ST2 would interfere with normal differentiation of 3T3-L1 preadipocytes; raising the possibility that it may affect normal differentiation of stromal preadipocytes and be a link to tumor metastatic capacity (AU)
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Colección: 06-national / ES Base de datos: IBECS Asunto principal: Neoplasias de la Mama / Células 3T3-L1 / Galactosa Tipo de estudio: Evaluation_studies Límite: Animals Idioma: En Revista: Clin. transl. oncol. (Print) Año: 2015 Tipo del documento: Article
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Colección: 06-national / ES Base de datos: IBECS Asunto principal: Neoplasias de la Mama / Células 3T3-L1 / Galactosa Tipo de estudio: Evaluation_studies Límite: Animals Idioma: En Revista: Clin. transl. oncol. (Print) Año: 2015 Tipo del documento: Article