IL6, IL10 and TGFB1 gene polymorphisms in coeliac disease: differences between DQ2 positive and negative patients
Allergol. immunopatol
; 33(5): 245-249, sept. 2005. tab
Article
en En
| IBECS
| ID: ibc-042133
Biblioteca responsable:
ES1.1
Ubicación: ES1.1 - BNCS
ABSTRACT
Predisposition to coeliac disease (CD) might be partially due to an individual pattern of hyper-inflammatory biased immune response. One of these patterns of intense response may be linked to the haplotype carrying HLA-DQ2 alleles and TNF-308A allele. However, 10 % of CD patients do not express the DQ2 heterodimer and these do not usually carry the TNF-308A allele. A similar response might be achieved by genes codifying other cytokines. Objectives: To study biallelic polymorphisms in genes codifying for TNFá, IL10, IL6 and TGFâ1 in DQ2 negative CD patients and to compare the results with DQ2 positive patients and healthy controls, in order to establish whether any of these polymorphisms have a role in CD susceptibility. Methods: TNF-308 (G > A), IL-6 174 (G > C) and TGFB1 codon 10 (+ 869, T > C) and codon 25 (+ 915, G > C) polymorphisms and IL-10 haplotype of polymorphisms in positions 1082 (G > A), 819 (C > T) and 592 (C > A) were typed by a SSP-PCR technique. Results: The distribution of allele frequencies for TNF 308 is different between DQ2 positive CD patients and controls and the same occurs for haplotype frequencies of the IL10 promoter (1082, 819, 592): The frequencies of the TNF-308A allele (p = 0.027), TNF 308A carriers (p = 0.031) and of IL10GCC haplotype are increased (p = 0.013) in DQ2 positive CD patients. However, the IL6 174 allele G is more frequent in DQ2 negative patients than in healthy controls (p = 0.018), DQ2 negative controls (p = 0,018), and DQ2 positive patients (p = 0.008). Conclusions: DQ2 negative CD patients show an increased frequency of genotypes associated to IL6 high production. These were mainly allele G homozygous for the IL6 gene (174) polymorphism. The IL6 174GG genotype (homozygous) may be an additional risk marker for CD in DQ2 negative patients, representing an alternative susceptibility factor for CD when TNF -308A is negative
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Colección:
06-national
/
ES
Base de datos:
IBECS
Asunto principal:
Antígenos HLA-DQ
/
Enfermedad Celíaca
/
Interleucina-6
/
Interleucina-10
Tipo de estudio:
Clinical_trials
Límite:
Humans
Idioma:
En
Revista:
Allergol. immunopatol
Año:
2005
Tipo del documento:
Article