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Mucosal immunization with PspA (Pneumococcal surface protein A)-adsorbed nanoparticles targeting the lungs for protection against pneumococcal infection
Rodrigues, Tasson Costa; Oliveira, Maria Leonor Sarno de; Schanoski, Alessandra Soares; Rico, Stefanni Liliane Chavez; Figueiredo, Douglas Borges; Gonçalves, Viviane Maimoni; Ferreira, Daniela M; Kunda, Nitesh K; Saleem, Imran Y; Miyaji, Eliane Namie.
Afiliación
  • Rodrigues, Tasson Costa; Instituto Butantan. Laboratório de Bacteriologia.
  • Oliveira, Maria Leonor Sarno de; Instituto Butantan. Laboratório de Bacteriologia.
  • Schanoski, Alessandra Soares; Instituto Butantan. Laboratório de Bacteriologia.
  • Rico, Stefanni Liliane Chavez; Instituto Butantan. Laboratório de Bacteriologia.
  • Figueiredo, Douglas Borges; Instituto Butantan. Laboratório Especial de Desenvolvimento de Vacinas (LEDV).
  • Gonçalves, Viviane Maimoni; Instituto Butantan. Laboratório Especial de Desenvolvimento de Vacinas (LEDV).
  • Ferreira, Daniela M; Instituto Butantan. Laboratório de Bacteriologia.
PLoS One ; 13(1): e0191692, 2018.
Article en En | SES-SP, SESSP-IBPROD, SES-SP | ID: but-ib14956
Biblioteca responsable: BR78.1
Ubicación: BR78.1
ABSTRACT
Burden of pneumonia caused by Streptococcus pneumoniae remains high despite the availability of conjugate vaccines. Mucosal immunization targeting the lungs is an attractive alternative for the induction of local immune responses to improve protection against pneumonia. Our group had previously described the development of poly(glycerol adipate-co-omega-pentadecalactone) (PGA-co-PDL) polymeric nanoparticles (NPs) adsorbed with Pneumococcal surface protein A from clade 4 (PspA4Pro) within L-leucine microcarriers (nanocomposite microparticles-NCMPs) for mucosal delivery targeting the lungs (NP/NCMP PspA4Pro). NP/NCMP PspA4Pro was now used for immunization of mice. Inoculation of this formulation induced anti-PspA4Pro IgG antibodies in serum and lungs. Analysis of binding of serum IgG to intact bacteria showed efficient binding to bacteria expressing PspA from clades 3, 4 and 5 (family 2), but no binding to bacteria expressing PspA from clades 1 and 2 (family 1) was observed. Both mucosal immunization with NP/NCMP PspA4Pro and subcutaneous injection of the protein elicited partial protection against intranasal lethal pneumococcal challenge with a serotype 3 strain expressing PspA from clade 5 (PspA5). Although similar survival levels were observed for mucosal immunization with NP/NCMP PspA4Pro and subcutaneous immunization with purified protein, NP/NCMP PspA4Pro induced earlier control of the infection. Conversely, neither immunization with NP/NCMP PspA4Pro nor subcutaneous immunization with purified protein reduced bacterial burden in the lungs after challenge with a serotype 19F strain expressing PspA from clade 1 (PspA1). Mucosal immunization with NP/NCMP PspA4Pro targeting the lungs is thus able to induce local and systemic antibodies, conferring protection only against a strain expressing PspA from the homologous family 2.
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Texto completo: 1 Colección: 06-national / BR Base de datos: SES-SP / SESSP-IBPROD Idioma: En Revista: PLoS One Año: 2018 Tipo del documento: Article
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google
Texto completo: 1 Colección: 06-national / BR Base de datos: SES-SP / SESSP-IBPROD Idioma: En Revista: PLoS One Año: 2018 Tipo del documento: Article