A biotechnological approach to immunotherapy: antivenom against Crotalus durissus cascavella snake venom produced from biodegradable nanoparticles
Int J Biol Macromol, v. 120, Part B, p. 1917-1924, dez. 2018
Article
en En
| SES-SP, SESSP-IBPROD, SES-SP
| ID: bud-2581
Biblioteca responsable:
BR78.1
ABSTRACT
Snakebite envenoming is a tropical disease neglected worldwide. In Brazil, the Crotalus durissus cascavella (CDC) snake belongs to a genus with venom of highest lethality. A search for new immunoadjuvants aimed to expand the therapeutic alternatives to improve vaccines and antivenom. This approach proposed to produce small and narrow-sized cationic CDC venom-loaded chitosan nanoparticles (CHNP) able to induce antibody response against the CDC venom. The ionic gelation method induced the formation of stable and slightly smooth spherical nanoparticles (<160?nm) with protein loading efficiency superior to 90%. The interactions between venom proteins and CHNP assessed using FT-IR spectroscopy corroborated with the in vitro release behavior of proteins from nanoparticles. Finally, the immunization animal model using BALB/c mice demonstrated the higher effectiveness of CDC venom-loaded CHNP compared to aluminum hydroxide, a conventional immunoadjuvant. Thus, CHNPs loaded with CDC venom exhibited a promising biotechnological approach to immunotherapy.
Texto completo:
1
Colección:
06-national
/
BR
Base de datos:
SES-SP
/
SESSP-IBPROD
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2018
Tipo del documento:
Article