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HPLC fingerprint and simultaneous quantitative analysis of phyllanthin and hypophyllanthin for identification and authentication of Phyllanthus niruri from related species
Nasrulloh, Roni; Rafi, Mohamad; Wahyuni, Wulan Tri; Shimma, Shuichi; Heryanto, Rudi.
Afiliación
  • Nasrulloh, Roni; Bogor Agricultural University. Faculty of Mathematics and Natural Sciences. Department of Chemistry. Bogor. ID
  • Rafi, Mohamad; Bogor Agricultural University. Faculty of Mathematics and Natural Sciences. Department of Chemistry. Bogor. ID
  • Wahyuni, Wulan Tri; Bogor Agricultural University. Faculty of Mathematics and Natural Sciences. Department of Chemistry. Bogor. ID
  • Shimma, Shuichi; Department of Biotechnology, Graduate School of Engineering, Osaka University. Graduate School of Engineering. Department of Biotechnology. Suita. JP
  • Heryanto, Rudi; Bogor Agricultural University. Faculty of Mathematics and Natural Sciences. Department of Chemistry. Bogor. ID
Rev. bras. farmacogn ; 28(5): 527-532, Sept.-Oct. 2018. tab, graf
Article en En | LILACS | ID: biblio-977730
Biblioteca responsable: BR1.1
ABSTRACT
Abstract A precise and accurate method for the identification and authentication of Phyllanthus niruri L. from P. debilis Klein ex Willd. and P. urinaria L., Phyllanthaceae, was developed using high-performance liquid chromatography. Chromatographic fingerprint analysis was combined with simultaneous quantification of phyllanthin and hypophyllanthin for the developed method. Phyllanthin and hypophyllanthin were successfully separated and quantified under this proposed method. The highest amount of phyllanthin and hypophyllanthin was found in P. niruri compared to P. debilis and P. urinaria. Fingerprint chromatogram of the three Phyllanthus species showed distinct profiles that these may be used to identify and authenticate each Phyllanthus species, which improved by marker compounds present in each species. The combination of chromatographic fingerprint analysis and discriminant analysis was successfully discriminated all three species, including P. niruri adulterated with P. debilis or P. urinaria. The method can be used for the identification and authentication of P. niruri from related species, such as P. debilis and P. urinaria.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Tipo de estudio: Diagnostic_studies Idioma: En Revista: Rev. bras. farmacogn Asunto de la revista: FARMACIA Año: 2018 Tipo del documento: Article País de afiliación: Indonesia / Japón Pais de publicación: Brasil
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Tipo de estudio: Diagnostic_studies Idioma: En Revista: Rev. bras. farmacogn Asunto de la revista: FARMACIA Año: 2018 Tipo del documento: Article País de afiliación: Indonesia / Japón Pais de publicación: Brasil