Differential immunohistochemical expression of type I collagen and matrix metalloproteinase 2 among major salivary glands of young and geriatric mice
J. appl. oral sci
; 26: e20170484, 2018. tab, graf
Article
en En
| LILACS, BBO
| ID: biblio-954527
Biblioteca responsable:
BR1.1
ABSTRACT
Abstract Objective This study aimed to demonstrate the immunohistochemical changes associated with MMP-2 and type 1 collagen separately for the first time in the major salivary glands (the parotid, submaxillary, and sublingual glands) that occur with aging in mice. Material and Methods Fourteen Balb/c white mice (50-80 g) were used in this study. The animals were divided into two equal groups. Group I consisted of young animals (2-month-old) (n=7) and Group II consisted of older animals (18-month-old) (n=7). After routine histological follow-ups, Hematoxylin-eosin (H&E), Masson's Trichrome staining and immunohistochemical staining was performed for type I collagen and MMP-2. Results We observed that there were age-related decreases in the number of acinar cells, increase in eosinophilic zymogen granules in cells, collagen accumulation in fibrotic areas and dilatation in interlobular ducts. Also, while type I collagen and MMP-2 immunoreactivity were moderate in the salivary glands of the young mice, they were high in the salivary glands of the old mice (p=0.001). In the H-score assessment, MMP-2 immunoreactivity was lower at a significant level in young mice than in old mice (p=0.001). Conclusions This study showed that anatomical, physiological and morphological abnormalities occur in all three major salivary glands as a natural consequence of aging.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
BBO
/
LILACS
Asunto principal:
Glándula Parótida
/
Glándula Sublingual
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Glándula Submandibular
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Metaloproteinasa 2 de la Matriz
/
Colágeno Tipo I
Límite:
Animals
Idioma:
En
Revista:
J. appl. oral sci
Asunto de la revista:
ODONTOLOGIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Turquía
Pais de publicación:
Brasil