Cadmium-induced apoptosis of Siberian tiger fibroblasts via disrupted intracellular homeostasis
Biol. Res
; 49: 1-14, 2016. ilus, graf
Article
en En
| LILACS
| ID: biblio-950868
Biblioteca responsable:
CL1.1
ABSTRACT
BACKGROUND:
Heavy metals can cause great harm to Siberian tigers in the natural environment. Cadmium (Cd2+) is an environmental contaminant that affects multiple cellular processes, including cell proliferation, differentiation, and survival. It has been shown to induce apoptosis in a variety of cell types and tissues.RESULTS:
We investigated the apoptotic effects of Cd2+ on Siberian tiger fibroblasts in vitro. Our research revealed the typical signs of apoptosis after Cd²+ exposure. Apoptosis was dose- (0-4.8 µM) and duration-dependent (12-48 h), and proliferation was strongly inhibited. Cd²+ increased the activity of caspase-3, -8, and -9 and disrupted calcium homeostasis by causing oxidative stress and mitochondrial dysfunction. It also increased K+ efflux and altered the mRNA levels of Bax, Bcl-2, caspase-3, caspase-8, Fas, and p53.CONCLUSIONS:
Our results suggest that Cd2+ triggers the apoptosis of Siberian tiger fibroblasts by disturbing intracellular homeostasis. These results will aid in our understanding of the effects of Cd2+ on Siberian tigers and in developing interventions to treat and prevent cadmium poisoning.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Cadmio
/
Apoptosis
/
Espacio Intracelular
/
Tigres
/
Fibroblastos
/
Homeostasis
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Biol. Res
Asunto de la revista:
BIOLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Chile