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Down regulation of KLK7 expression in breast tissues and identification of a novel spliced KLK7 mRNA
Ejaz, Samina; Nasim, Faiz-ul-Hassan; Ashraf, Muhammad; Ahmad, Gulzar.
Afiliación
  • Ejaz, Samina; The Islamia University of Bahawalpur. Department of Chemistry. Bahawalpur. PK
  • Nasim, Faiz-ul-Hassan; The Islamia University of Bahawalpur. Department of Biochemistry and Biotechnology. Bahawalpur. PK
  • Ashraf, Muhammad; The Islamia University of Bahawalpur. Department of Chemistry. Bahawalpur. PK
  • Ahmad, Gulzar; Bahawal Victoria Hospital. Quaid-i-Azam Medical College. Department of Surgery. Bahawalpur. PK
Appl. cancer res ; 37: 1-13, 2017. tab, ilus
Article en En | LILACS, Inca | ID: biblio-914935
Biblioteca responsable: BR30.1
ABSTRACT

Background:

Alternative splicing commonly occurs in cancer cells and many cancer specific splice variants have been reported as potential candidate biomarkers of the disease. We have studied human tissue Kallikrein 7 (KLK7) mRNA expression profile in breast cancer patients of our region. KLK7 is member of a multi-gene family consisting of 15 members (KLK1-KLK15).

Methods:

We optimized touch down nested PCR method for the amplification of KLK7 isoforms/variants. Various bioinformatics tools were used for sequence analysis, identification of splicing pattern and prediction of encoded proteins.

Results:

We observed an unusual splicing event consisting of exon 3 (E3) truncation at 3' end (by 124 nucleotides), exon 4 (E4) exclusion and exon 5 (E5) truncation at 5' end (by 33 nucleotide) in 2 normal breast tissues, one obtained from invasive ductal carcinoma grade II patient and other collected from mammary dysplasia patient. Moreover, 3 other KLK7 mRNAs (KF963190, KF963191, and KF963193) expressed in breast cancer were noticed to exhibit single nucleotide polymorphism (SNPs). Bioinformatic analysis revealed that the alternatively spliced mRNA (KF963192) will potentially encode a truncated and non-functional protein. Similarly although encoded proteins have considerable homology with normal hK7 protein, SNPs seem to cause great variations in pIs, structures and molecular weights of encoded proteins.

Conclusions:

There is need to further explore the impact of the unique splicing event, SNPs and characterize these population specific mutations and their possible role in the pathogenesis of breast cancer (AU)
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS / Inca Asunto principal: Neoplasias de la Mama / Biomarcadores de Tumor / Regulación hacia Abajo / Isoformas de Proteínas Tipo de estudio: Diagnostic_studies Límite: Adolescent / Adult / Aged / Child / Child, preschool / Infant / Newborn / Pregnancy Idioma: En Revista: Appl. cancer res Asunto de la revista: NEOPLASIAS Año: 2017 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS / Inca Asunto principal: Neoplasias de la Mama / Biomarcadores de Tumor / Regulación hacia Abajo / Isoformas de Proteínas Tipo de estudio: Diagnostic_studies Límite: Adolescent / Adult / Aged / Child / Child, preschool / Infant / Newborn / Pregnancy Idioma: En Revista: Appl. cancer res Asunto de la revista: NEOPLASIAS Año: 2017 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Brasil