Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats
Acta cir. bras
; 32(1): 46-55, Jan. 2017. tab, graf
Article
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| LILACS
| ID: biblio-837671
Biblioteca responsable:
BR1.1
ABSTRACT
Abstract Purpose:
To evaluate the effect of oxacillin bonded to magnetic nanoparticles in local infection model in rat.Methods:
Twelve Wistar rats weighing 290±18g were randomly divided into four groups (n=6, each) and all rats had a magnet ring sutured on their right thighs. In the biodistribution group rats 0.1mL of 99mTc-magnetite (0.66 MBq) was injected i.v and after 30 minutes, biodistribution of 99mTc-magnetite was evaluated in right and left thighs. The other groups were inoculated with MRSA in each thigh muscles. Group 1 rats were injected i.v. with magnetite, group 2 with Magnetite + Oxacillin, group 3 with saline twice a day. After 24 hours samples of muscle secretion were harvested for microbiological analysis; muscle, lungs and kidneys for histology.Results:
99mTc-magnetite uptake was three-fold higher in right thigh muscles (with external magnet) than in the left. In magnetite and oxacillin-magnetite groups, bacterial/CFU was significantly lower in thigh muscles than in saline-controls. The inflammatory reaction in muscles and lungs was significantly lower in oxacillin-magnetite group-rats than in other groups (p<0.001) .Conclusion:
This study confirms the potential antimicrobial activity of magnetic nanoparticles for Methicillin-Resistant S. aureus strains, which in addition to concentrate the antibiotic at the infection site, positively influenced the treatment.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Oxacilina
/
Infecciones Estafilocócicas
/
Staphylococcus aureus Resistente a Meticilina
/
Nanopartículas de Magnetita
/
Antibacterianos
Límite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Asunto de la revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Año:
2017
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil