Your browser doesn't support javascript.
loading
Insecticide resistance levels and associated mechanisms in three Aedes aegypti populations from Venezuela
Rubio-Palis, Yasmin; Dzuris, Nicole; Sandi, Christopher; Vizcaino-Cabarrus, Rita Lucrecia; Corredor-Medina, Claudia; González, Jesús Alberto; Lenhart, Audrey E.
Afiliación
  • Rubio-Palis, Yasmin; Universidad de Carabobo. Instituto de Investigaciones Biomédicas. Maracay. VE
  • Dzuris, Nicole; US Centers for Disease Control and Prevention. Center for Global Health. Division of Parasitic Diseases and Malaria, Entomology Branch. Atlanta. US
  • Sandi, Christopher; US Centers for Disease Control and Prevention. Center for Global Health. Division of Parasitic Diseases and Malaria, Entomology Branch. Atlanta. US
  • Vizcaino-Cabarrus, Rita Lucrecia; US Centers for Disease Control and Prevention. Center for Global Health. Division of Parasitic Diseases and Malaria, Entomology Branch. Atlanta. US
  • Corredor-Medina, Claudia; US Centers for Disease Control and Prevention. Center for Global Health. Division of Parasitic Diseases and Malaria, Entomology Branch. Atlanta. US
  • González, Jesús Alberto; Ministerio del Poder Popular para la Salud. Dirección General de Salud Ambiental. Dirección de Control de Vectores. Maracay. VE
  • Lenhart, Audrey E; US Centers for Disease Control and Prevention. Center for Global Health. Division of Parasitic Diseases and Malaria, Entomology Branch. Atlanta. US
Mem. Inst. Oswaldo Cruz ; 118: e220210, 2023. tab, graf
Article en En | LILACS-Express | LILACS | ID: biblio-1440666
Biblioteca responsable: BR1.1
ABSTRACT
BACKGROUND The massive use of insecticides in public health has exerted selective pressure resulting in the development of resistance in Aedes aegypti to different insecticides in Venezuela. Between 2010 and 2020, the only insecticides available for vector control were the organophosphates (Ops) fenitrothion and temephos which were focally applied. OBJECTIVES To determine the state of insecticide resistance and to identify the possible biochemical and molecular mechanisms involved in three populations of Ae. aegypti from Venezuela. METHODS CDC bottle bioassays were conducted on Ae. aegypti collected between October 2019 and February 2020 in two hyperendemic localities for dengue in Aragua State and in a malaria endemic area in Bolívar State. Insecticide resistance mechanisms were studied using biochemical assays and polymerase chain reaction (PCR) to detect kdr mutations. FINDINGS Bioassays showed contrasting results among populations; Las Brisas was resistant to malathion, permethrin and deltamethrin, Urbanización 19 de Abril was resistant to permethrin and Nacupay to malathion. All populations showed significantly higher activity of mixed function oxidases and glutathione-S-transferases (GSTs) in comparison with the susceptible strain. The kdr mutations V410L, F1534C, and V1016I were detected in all populations, with F1534C at higher frequencies. MAIN CONCLUSION Insecticide resistance persists in three Ae. aegypti populations from Venezuela even in the relative absence of insecticide application.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Tipo de estudio: Prognostic_studies / Risk_factors_studies País/Región como asunto: America do sul / Venezuela Idioma: En Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: MEDICINA TROPICAL / PARASITOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos / Venezuela Pais de publicación: Brasil
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Tipo de estudio: Prognostic_studies / Risk_factors_studies País/Región como asunto: America do sul / Venezuela Idioma: En Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: MEDICINA TROPICAL / PARASITOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos / Venezuela Pais de publicación: Brasil