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Evaluation of larval surface antigens from infective larvae of Strongyloides venezuelensis for the serodiagnosis of human strongyloidiasis
Gomes, Bruna Barroso; Gonzales, William Henry Roldan; Meisel, Dirce Mary Correa; Gryschek, Ronaldo Cesar Borges; Paula, Fabiana Martins de.
Afiliación
  • Gomes, Bruna Barroso; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Gonzales, William Henry Roldan; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Meisel, Dirce Mary Correa; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Gryschek, Ronaldo Cesar Borges; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
  • Paula, Fabiana Martins de; Universidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. São Paulo. BR
Rev. Inst. Med. Trop. São Paulo (Online) ; 65: e1, 2023. tab, graf
Article en En | LILACS-Express | LILACS | ID: biblio-1422789
Biblioteca responsable: BR1.1
ABSTRACT
ABSTRACT Serodiagnosis of strongyloidiasis is usually performed by ELISA for the detection of IgG antibodies due to its high sensitivity and practicality, but its main limitation is a constant source of S. stercoralis antigens. The use of S. venezuelensis as a heterologous source of antigens has facilitated several published studies on the serodiagnosis and epidemiology of human strongyloidiasis. The main objective of this study was to evaluate the diagnostic accuracy of surface cuticle antigens of infective larvae of S. venezuelensis extracted with CTAB detergent (L3-CTAB) in comparison with soluble somatic extracts (L3-SSE) using a panel of sera from immunocompetent and immunocompromised individuals, at three different cut-offs. ROC curve analysis showed that L3-CTAB had an AUC of 0.9926. At the first cut-off value (OD 450 nm = 0.214), sensitivity and specificity were 100% and 90.11%, respectively, with a diagnostic accuracy of 0.93. At a second cut-off value (OD 450 nm = 0.286), sensitivity and specificity were 70% and 100%, respectively, with a diagnostic accuracy of 0.91. However, at an alternative third cut-off value (OD 450 nm = 0.589), sensitivity and specificity were 95% and 97.8%, respectively, with a diagnostic accuracy of 0.97. Using L3-CTAB as an antigenic source, the seropositivity rate in immunocompromised patients was 28.13% (9/32) whereas a seropositivity rate of 34.38% (11/32) was found when L3-SSE was used in ELISA. Therefore, the L3-CTAB is simple and practical to obtain and was found to be highly sensitive and specific.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Tipo de estudio: Prognostic_studies Idioma: En Revista: Rev. Inst. Med. Trop. São Paulo (Online) Asunto de la revista: Medicina Tropical Año: 2023 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Tipo de estudio: Prognostic_studies Idioma: En Revista: Rev. Inst. Med. Trop. São Paulo (Online) Asunto de la revista: Medicina Tropical Año: 2023 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil