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Extraction optimization, Total Phenolic-Flavonoids content, HPLC-DAD finger printing, antimicrobial, antioxidant and cytotoxic potentials of Chinese folklore Ephedra intermedia Schrenk & C. A. Mey
Nasar, Muhammad Qasim; Zohra, Tanzeel; Khalil, Ali Talha; Ovais, Muhammad; Ullah, Ikram; Ayaz, Muhammad; Zahoor, Mohammad; Shinwari, Zabta Khan.
Afiliación
  • Nasar, Muhammad Qasim; Quaid-i-Azam University. Department of Biotechnology. Islamabad. PK
  • Zohra, Tanzeel; Quaid-i-Azam University. Department of Biotechnology. Islamabad. PK
  • Khalil, Ali Talha; Lady Reading Hospital (MTI). Molecular Biology and pathology Department. Peshawar. PK
  • Ovais, Muhammad; National Center for Nanoscience and Technology. CAS Center for Excellence in Nanoscience. Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety. Beijing. CN
  • Ullah, Ikram; Quaid-i-Azam University. Department of Biotechnology. Islamabad. PK
  • Ayaz, Muhammad; University of Malakand. Department of Pharmacy. Khyber Pakhtunkhwa. PK
  • Zahoor, Mohammad; University of Malakand. Department of Chemistry. PK
  • Shinwari, Zabta Khan; Quaid-i-Azam University. Department of Biotechnology. Islamabad. PK
Braz. J. Pharm. Sci. (Online) ; 58: e20989, 2022. tab, graf
Article en En | LILACS-Express | LILACS | ID: biblio-1420394
Biblioteca responsable: BR1.1
ABSTRACT
Abstract Plants from genus Ephedra are commonly used by the Chinese people as folk medicine for treatment of various diseases. The current study was designed to explore the ethno-pharmacological based pharmacological potentials of Ephedra intermedia Schrenk & C.A. Mey. (E. intermedia). Plant aerial parts were extracted using ten solvent systems with increasing order of polarity. Samples were analyzed for total phenolic and flavonoid contents, HPLC-DAD analysis, antibacterial, antifungal, HepG2 cell line cytotoxicity, hemolysis and antioxidant potentials following standard procedures. Highest percent extract recovery was observed in Eth+WT (25.55 % w/w) solvent system. Flavonoid and phenolic contents were higher in chloroform and Met+WT fractions respectively. Considerable antibacterial activity was shown by Eth+Met extract against B. subtilis and K. pneumonia (MIC of 11.1μg/mL for each). Eth extract exhibited high antifungal activity against A. fumigates (15±0.31 mm DIZ). Met+WT extract showed significant cytotoxicity against HepG2 cell lines with IC50 of 13.51+0.69 μg/mL. Substantial free radical scavenging activity (74.9%) was observed for Met+Eth extract. In the current study, several solvent systems were used for more effective extraction of fractions and can be useful in the isolation of phytochemicals. Various fractions exhibited considerable antimicrobial, antioxidant and cytotoxic potentials. Biological potentials of E. intermedia signify its potential uses in microbial, cancer and degenerative disorders and thus warrant further detailed studies.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Idioma: En Revista: Braz. J. Pharm. Sci. (Online) Asunto de la revista: Farmacologia / Terapˆutica / Toxicologia Año: 2022 Tipo del documento: Article País de afiliación: China / Pakistán Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Idioma: En Revista: Braz. J. Pharm. Sci. (Online) Asunto de la revista: Farmacologia / Terapˆutica / Toxicologia Año: 2022 Tipo del documento: Article País de afiliación: China / Pakistán Pais de publicación: Brasil