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Immunoanalysis of different antigenic preparations of Angiostrongylus cantonensis for neuroangiostrongyliasis diagnosis improvement
Melo, Leyva Cecília Vieira de; Souza, Felipe Corrêa Rezende de; Baccin, Amanda de Oliveira; Mota, Dan Jessé Gonçalves da; Pereira-Chioccola, Vera Lucia; Pinto, Pedro Luiz Silva.
Afiliación
  • Melo, Leyva Cecília Vieira de; Instituto Adolfo Lutz. Centro de Parasitologia e Micologia. Núcleo de Enteroparasitas. São Paulo. BR
  • Souza, Felipe Corrêa Rezende de; Instituto Adolfo Lutz. Centro de Parasitologia e Micologia. Núcleo de Enteroparasitas. São Paulo. BR
  • Baccin, Amanda de Oliveira; Instituto Adolfo Lutz. Centro de Parasitologia e Micologia. Núcleo de Enteroparasitas. São Paulo. BR
  • Mota, Dan Jessé Gonçalves da; Unidade de Vigilância em Saúde. São Paulo. BR
  • Pereira-Chioccola, Vera Lucia; Secretaria Estadual da Saúde de São Paulo. Coordenadoria do Controle de Doenças. Programa de Pós-Graduação. São Paulo. BR
  • Pinto, Pedro Luiz Silva; Instituto Adolfo Lutz. Centro de Parasitologia e Micologia. Núcleo de Enteroparasitas. São Paulo. BR
Mem. Inst. Oswaldo Cruz ; 117: e220086, 2022. tab, graf
Article en En | LILACS-Express | LILACS | ID: biblio-1405993
Biblioteca responsable: BR1.1
ABSTRACT
BACKGROUND Angiostrongylus cantonensis is the etiological agent of neuroangiostrongyliasis in humans, which is developed in gastropods and vertebrate species, mainly rodents. Human transmission occurs through consumption of molluscs and paratenic hosts infected with L3, and the migration of larvae to the central nervous system causes eosinophilic meningitis. Laboratory diagnosis is based on molecular and immunological tests, using young or adult females as a source of antigens. However, these tests give positive results only after several weeks of symptoms onset and also cross-reactions with others parasite infections may occur. OBJECTIVES The purpose of this work was to study different antigenic preparations of distinct evolutionary phases of A. cantonensis, in order to improve serological techniques for disease immunodiagnosis. METHODS For this purpose, antigenic fractions of different evolutionary forms were evaluated by Dot-enzyme-linked immunosorbent assay (Dot-ELISA) and Western blot using serum samples. FINDINGS All analysed fractions showed reactivity with serum samples from patients with neuroangiostrongyliasis, especially female membrane alkaline (FAM) and female soluble alkaline (FAS) fractions together with female soluble saline (FSS), improving the technique specificity. MAIN CONCLUSIONS The results point to the possibility of use of raw female antigens in association with alkaline membrane antigens extracted from adult worms to aid in diagnosis and helps initiate neuroangiostrongyliasis surveillance and control actions.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Tipo de estudio: Diagnostic_studies Idioma: En Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: MEDICINA TROPICAL / PARASITOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Tipo de estudio: Diagnostic_studies Idioma: En Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: MEDICINA TROPICAL / PARASITOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil