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Immunohistochemistry of resorption and inflammation factors in the periodontal ligament of human deciduous teeth
Santos, Bianca Zimmermann dos; Dutra, Rafael Cypriano; Santos, Adair Roberto Soares dos; Casarin, Maísa; Goldfeder, Eliane Maria; Bosco, Vera Lucia; Cordeiro, Mabel Mariela Rodriguez.
Afiliación
  • Santos, Bianca Zimmermann dos; Universidade Franciscana. School of Dentistry. Santa Maria. BR
  • Dutra, Rafael Cypriano; Universidade Federal de Santa Catarina. Department of Health Science. Araranguá. BR
  • Santos, Adair Roberto Soares dos; Universidade Federal de Santa Catarina. Department of Physiological Sciences. Araranguá. BR
  • Casarin, Maísa; Universidade Federal de Pelotas. Department of Semiology and Clinic. Pelotas. BR
  • Goldfeder, Eliane Maria; Universidade Federal de Santa Catarina. Centre for Biological Sciences. Department of Morphological Sciences. Florianópolis. BR
  • Bosco, Vera Lucia; Universidade Federal de Santa Catarina. Centre of Health Sciences. Department of Dentistry. Florianópolis. BR
  • Cordeiro, Mabel Mariela Rodriguez; Universidade Federal de Santa Catarina. Department of Dentistry. Florianópolis. BR
Braz. oral res. (Online) ; 36: e056, 2022. graf
Article en En | LILACS-Express | LILACS, BBO | ID: biblio-1374757
Biblioteca responsable: BR1.1
ABSTRACT
Abstract The understanding of the biological mechanisms involved in root resorption in deciduous teeth is important to the future development of preventive measures and treatments of this condition. The aim of the present study was to compare the expression and immunostaining of iNOS, MMP-9, OPG and RANKL in the periodontal ligament (PDL) of deciduous teeth with physiologic root resorption (GI), inflammatory pathological root resorption (GII) and permanent teeth (GIII), the negative control. Teeth in GI (n = 10), GII (n = 10) and (GIII) (n = 10) were submitted to immunohistochemical analysis to determine the expression of iNOS, MMP-9, OPG, and RANKL. The immunostaining was analysed by optical density. Statistical analysis included one-way ANOVA, followed by Student-Newman-Keuls post hoc test (p < 0.05). The results showed that iNOS, MMP-9 and RANKL expression in the PDL was higher in GII compared to GI and GIII (p < 0.05). Moreover, RANKL expression was higher in GI compared to GIII (p < 0.001), while OPG immunolabelling was lower in GII compared to GI and GIII (p < 0.001). The PDL of deciduous teeth bearing inflammatory processed exhibited upregulation of resorption-associated factors as well as enzymes related to tissue degradation which, in turn explains the exacerbation and greater susceptibility of those teeth to root resorption process.
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Texto completo: 1 Colección: 01-internacional Base de datos: BBO / LILACS Idioma: En Revista: Braz. oral res. (Online) Asunto de la revista: ODONTOLOGIA Año: 2022 Tipo del documento: Article / Project document País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: BBO / LILACS Idioma: En Revista: Braz. oral res. (Online) Asunto de la revista: ODONTOLOGIA Año: 2022 Tipo del documento: Article / Project document País de afiliación: Brasil Pais de publicación: Brasil