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Serological and Molecular Detection of Latent Viruses in the Apple Germplasm Bank of Santa Catarina
Manenti, Danielle Caroline; Hawerroth, Maraisa Crestani; Brancher, Thyana Lays; Schuh, Filipe Schmidt; Kvitschal, Marcus Vinicius; Nickel, Osmar; Souto, Eliezer Rodrigues de.
Afiliación
  • Manenti, Danielle Caroline; State University of Maringá. Agronomy Department. Maringá. BR
  • Hawerroth, Maraisa Crestani; Epagri. Experimental Station of Caçador. Caçador. BR
  • Brancher, Thyana Lays; Epagri. Experimental Station of Caçador. Caçador. BR
  • Schuh, Filipe Schmidt; State University of Maringá. Agronomy Department. Maringá. BR
  • Kvitschal, Marcus Vinicius; Epagri. Experimental Station of Caçador. Caçador. BR
  • Nickel, Osmar; Embrapa Grape and Wine. Bento Gonçalves. BR
  • Souto, Eliezer Rodrigues de; State University of Maringá. Agronomy Department. Maringá. BR
Braz. arch. biol. technol ; Braz. arch. biol. technol;63: e20190272, 2020. tab, graf
Article en En | LILACS | ID: biblio-1132258
Biblioteca responsable: BR1.1
ABSTRACT
Abstract The Apple Germplasm Bank (AGB) of Santa Catarina Agricultural Research and Rural Extension Company - Epagri, AGB-Epagri, is the largest of the genus Malus in Brazil. Twenty-eight main accessions of this bank were virus screened through DAS-ELISA, RT-PCR and IC-RT-PCR during two consecutive reproductive cycles, and each accession showed latent mixed infection by at least two species, among ASGV, ASPV and ACLSV. The combined use of diagnostic methods helped overcome inconsistencies commonly found in apple virus detection and was shown essential for the AGB-Epagri can be safely used as a source of genetic variability and for the exchange of virus-free propagative material.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Malus / Flexiviridae / Banco de Semillas Tipo de estudio: Diagnostic_studies Idioma: En Revista: Braz. arch. biol. technol Asunto de la revista: BIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil

Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Malus / Flexiviridae / Banco de Semillas Tipo de estudio: Diagnostic_studies Idioma: En Revista: Braz. arch. biol. technol Asunto de la revista: BIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil