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Detection of Plasmodium sp. in capybara
Santos, LC; Curotto, SM; Moraes, W; Cubas, ZS; Costa-Nascimento, MJ; Barros-Filho, IR; Biondo, AW; Kirchgatter, K.
Afiliación
  • Santos, LC; Environmental Laboratory. Foz do Iguassu. BR
  • Curotto, SM; Federal University of Parana. Post-Graduation Course in Veterinary Sciences. Curitiba. BR
  • Moraes, W; Bela Vista Biological Sanctuary. Foz do Iguassu. BR
  • Cubas, ZS; Bela Vista Biological Sanctuary. Foz do Iguassu. BR
  • Costa-Nascimento, MJ; Superintendency for Endemic Disease Control/Tropical Medicine Institute of Sao Paulo. Malaria Research Center. São Paulo. BR
  • Barros-Filho, IR; University of Parana. Veterinary Medicine Department. Curitiba. BR
  • Biondo, AW; University of Parana. Veterinary Medicine Department. Curitiba. BR
  • Kirchgatter, K; Superintendency for Endemic Disease Control/Tropical Medicine Institute of Sao Paulo. Malaria Research Center. São Paulo. BR
Vet. parasitol ; Vet. parasitol;163(1-2): 148-151, 7 july 2009.
Article en En | SES-SP, SESSP-SUCENPROD, SES-SP | ID: biblio-1068390
Biblioteca responsable: BR93.2
Ubicación: BR93.2
ABSTRACT
In the present study, we have microscopically and molecularly surveyed blood samples from 11 captive capybaras (Hydrochaeris hydrochaeris) from the Sanctuary Zoo for Plasmodium sp. infection. One animal presented positive on blood smear by light microscopy. Polymerase chain reaction was carried out accordingly using a nested genus-specific protocol, which uses oligonucleotides from conserved sequences flanking a variable sequence region in the small subunit ribosomal RNA (ssrRNA) of all Plasmodium organisms. This revealed three positive animals. Products from two samples were purified and sequenced. The results showed less than 1% divergence between the two capybara sequences. When compared with GenBank sequences, a 55% similarity was obtained to Toxoplasma gondii and a higher similarity (73–77.2%) was found to ssrRNAs from Plasmodium species that infect reptile, avian, rodents, and human beings. The most similar Plasmodium sequence was from Plasmodium mexicanum that infects lizards of North America, where around 78% identity was found...
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Texto completo: 1 Colección: 06-national / BR Base de datos: SES-SP / SESSP-SUCENPROD Asunto principal: Plasmodium Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Vet. parasitol Año: 2009 Tipo del documento: Article
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 06-national / BR Base de datos: SES-SP / SESSP-SUCENPROD Asunto principal: Plasmodium Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Vet. parasitol Año: 2009 Tipo del documento: Article