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Identification of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus actively infesting dogs
Viol, Milena Araúz; Guerrero, Felix D; Costa de Aquino, Monally Conceição; Loiola, Saulo Hudson; Dias de Melo, Guilherme; Gomes, Aparecida Helena de Souza; Kanamura, Cristina Takami; Garcia, Marcos Valério; Andreotti, Renato; Lima, Valéria Marçal Félix de; Bresciani, Katia Denise Saraiva.
Afiliación
  • Viol, Milena Araúz; Universidade Estadual Paulista. BR
  • Guerrero, Felix D; Knipling-Bushland U. S. Livestock Insects Research LaboratoryKerrville. US
  • Costa de Aquino, Monally Conceição; Universidade Estadual Paulista. BR
  • Loiola, Saulo Hudson; Universidade Estadual Paulista. BR
  • Dias de Melo, Guilherme; Universidade Estadual Paulista. BR
  • Gomes, Aparecida Helena de Souza; Secretaria da Saúde do Estado de São Paulo. Instituto Adolfo Lutz. BR
  • Kanamura, Cristina Takami; Secretaria da Saúde do Estado de São Paulo. Instituto Adolfo Lutz. BR
  • Garcia, Marcos Valério; Empresa Brasileira de Pesquisa Agropecuária. BR
  • Andreotti, Renato; Empresa Brasileira de Pesquisa Agropecuária. BR
  • Lima, Valéria Marçal Félix de; Universidade Estadual Paulista. BR
  • Bresciani, Katia Denise Saraiva; Universidade Estadual Paulista. BR
Parasitology Research ; 115: 3479-3484, 2016.
Article en En | LILACS, SES-SP | ID: biblio-1024756
Biblioteca responsable: BR91.2
ABSTRACT
Sand flies are recognized as the major vector of canine visceral leishmaniasis. However, in some areas of Brazil where sand flies do not occur, this disease is found in humans and dogs. There has been speculation that ticks might play a role in transmission of canine visceral leishmaniasis and the DNA of Leishmania spp. has been reported in whole ticks. We investigated the presence of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus ticks collected from tick-infested dogs in two cities of Brazil. We used 66 dogs that tested positive and 33 that tested negative for Leishmania spp. according to direct cytological examination assays. Ten ticks were collected from each dog and dissected to collect the intestines, ovaries, and salivary glands for immunohistochemistry (IHC) and diagnostic real-time polymerase chain reaction (RT-PCR). IHC results showed Leishmania spp. in 98, 14, and 8 % of the intestines, ovaries, and salivary glands, respectively. Real-time PCR showed that 89, 41, and 33 % of the tick intestine, ovary, and salivary glands, respectively, were positive for Leishmania spp. The verification of promastigotes of Leishmania spp. by two independent techniques in ticks collected from these urban region dogs showed that there is need for clarification of the role of ticks in the transmission of canine visceral leishmaniasis in Brazil.
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS / SES-SP Asunto principal: Ovario / Rhipicephalus / Leishmania Tipo de estudio: Diagnostic_studies Idioma: En Revista: Parasitology Research Año: 2016 Tipo del documento: Article País de afiliación: Brasil / Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
Buscar en Google
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS / SES-SP Asunto principal: Ovario / Rhipicephalus / Leishmania Tipo de estudio: Diagnostic_studies Idioma: En Revista: Parasitology Research Año: 2016 Tipo del documento: Article País de afiliación: Brasil / Estados Unidos