Cytotoxicity of cannulas for ophthalmic surgery after cleaning and sterilization: evaluation of the use of enzymatic detergent to remove residual ophthalmic viscosugical device material
Journal of Cataract & Refractive Surgery
; 39: 937-941, 2013. ilus
Article
en En
| SES-SP, LILACS, SESSP-IALPROD, SES-SP, SESSP-IALACERVO
| ID: biblio-1017138
Biblioteca responsable:
BR91.2
Ubicación: BR76.1; P
ABSTRACT
PURPOSE:
To evaluate the cytotoxicity of reusable cannulas for ophthalmic surgery after the cannulas were filled with an ophthalmic viscosurgical device (OVD) and cleaned with an enzymatic detergent.SETTING:
Microbiological Testing Laboratory, Department of Medical-Surgical Nursing, University of S~ao Paulo School of Nursing, and Cell Culture Section, Adolfo Lutz Institute, S~ao Paulo, Brazil.DESIGN:
Experimental study.METHODS:
The sample consisted of 30 reusable 25-gauge injection cannulas, 20.0 mm in length, whose lumens were filled with an OVD solution for 50 minutes. The following steps were used to process the cannulas (1) presoaking, (2) washing the lumen using a high-pressure water jet, (3) backwashing with enzymatic detergent in ultrasonic cleaner, (4) preliminary rinsing with tap water, (5) final rinsing with sterile distilled water, (6) drying with compressed filtered air, (7) wrapping in surgical-grade paper, and (8) steam sterilization at 134C for 4 minutes. The cannulas were then tested for cytotoxicity according to the United States Pharmacopeia 32.RESULTS:
The cleaning protocol used in this study removed residues of OVD solution and enzymatic detergent as shown by the lack of cytotoxicity of all sample extracts.CONCLUSION:
This cleaning protocol has the potential to minimize the occurrence of toxic anterior segment syndrome associated with residues of OVD solutions and enzymatic detergents. Financial Disclosure No author has a financial or proprietary interest in any material or method mentioned.
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
/
SES-SP
/
SESSP-IALACERVO
/
SESSP-IALPROD
Asunto principal:
Cirugía General
/
Agua
/
Esterilización
/
Tareas del Hogar
Idioma:
En
Revista:
Journal of Cataract & Refractive Surgery
Año:
2013
Tipo del documento:
Article
País de afiliación:
Brasil