RESUMO
BACKGROUND: Prostate-specific antigen (PSA) has been used for the diagnosis and follow up of prostate cancer (PCa). METHODS: Mouse monoclonal antibodies (MAbs) were generated against human prostate-specific antigen (PSA) for the development of a sensitive total PSA (t-PSA) assay. Two MAbs, denoted CB-PSA.4 and CB-PSA.9, with affinities of 3.7 x 10(9) and 4.7 x 10(10) l/mol, respectively, were used to develop an enzyme-linked immunosorbent assay (ELISA) for quantifying serum t-PSA concentration. RESULTS: The detection limit (DL) of the assay was 0.1 microg/l (n=20, mean of "zero" standard+3S.D.), and the recovery of t-PSA was 96-103%. The within-run and between-day coefficients of variation (CV) ranged from 2.1% to 3.2%, and from 2.8% to 6.3% for PSA concentrations of 10 and 1 microg/l, respectively. The equimolar detection of t-PSA and free-PSA was demonstrated by two different methods, one consisted in the comparative evaluation of a sera panel (n=9) with our enzyme-linked immunosorbent assay (ELISA) and four commercial total PSA assays and the concordance with CIS bio total PSA assay. The assay had a linear range of 0.12 to 25 microg/l. CONCLUSIONS: The analytical performance characteristics of our PSA ELISA suggest that it will provide clinically useful PSA results, particularly when diagnostic algorithms are used.
Assuntos
Ensaio de Imunoadsorção Enzimática/métodos , Antígeno Prostático Específico/sangue , Animais , Anticorpos Monoclonais , Ensaio de Imunoadsorção Enzimática/normas , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Antígeno Prostático Específico/imunologia , Neoplasias da Próstata/diagnóstico , alfa 1-Antiquimotripsina/análise , alfa 1-Antiquimotripsina/metabolismoRESUMO
BACKGROUND: Malignant fibrous histiocytoma (MFH) arising primarily in the lungs is rare, and a preoperative diagnosis, as well as a surgical planning, is very important because of the tumor's propensity for vascular invasion and its low incidence of lymph node metastasis. The correct preoperative diagnosis of thoracic MFH is not easy to establish because the small fragments from needle and transbronchial biopsies are often inadequate for a conclusive histologic analysis. A preoperative bronchial brushing cytology suggestion of the diagnosis of primary MFH of the lungs may be helpful in such cases. CASE: A 37-year-old male presented with a large, irregular mass in the inferior and middle lobes on chest roentgenography as well as on computed tomography. Two bronchoscopies were performed, with the diagnosis of undifferentiated large cell carcinoma. After surgical resection a more sophisticated pathologic analysis, including immunohistochemical and ultrastructural studies, revealed a primary MFH of the lungs. Revision of the bronchial brushing cytology disclosed many spindle-shaped cells with a "comet" configuration, strongly suggestive of MFH. CONCLUSION: The bronchial brushing cytology features of spindle-shaped and bizarre, multinucleated giant cells with a comet appearance may be the key to the cytomorphologic diagnosis of MFH.
Assuntos
Histiocitoma Fibroso Benigno/diagnóstico , Neoplasias Pulmonares/diagnóstico , Adulto , Biomarcadores/análise , Técnicas Citológicas , Histiocitoma Fibroso Benigno/química , Histiocitoma Fibroso Benigno/patologia , Humanos , Neoplasias Pulmonares/química , Neoplasias Pulmonares/patologia , Masculino , Microscopia Eletrônica , alfa 1-Antiquimotripsina/análiseRESUMO
OBJECTIVE: To study the nature of multinucleated and mononuclear cells from peripheral giant cell granuloma (PGCG). MATERIALS AND METHODS: Formalin-fixed, paraffin-embedded sections of 40 cases of PGCG were immunohistochemically stained for vimentin, alpha I-antichymotrypsin, CD68, S-100 protein, lysozyme, leucocyte common antigen (LCA), factor VIII-related antigen and muscle cell actin. Six cases of PGCG were also studied by transmission electron microscopy. RESULTS: Vimentin, alpha I-antichymotrypsin and CD68 were expressed in both the mononuclear and multinucleated giant cells. Dendritic mononuclear cells, positive for S-100 protein, were noted in 67.5% of the lesions, whereas lysozyme and leucocyte common antigen were detected in occasional mononuclear cells. Ultrastructural examination showed mononuclear cells with signs of phagocytosis and sometimes interdigitations with similar cells. Others presented non-specific characteristics and the third type exhibited cytoplasmic processes and occasional Birbeck granules. Some multinucleated giant cells showed oval nuclei, abundant mitochondria and granular endoplasmic reticulum whereas others presented with irregular nuclei and a great number of cytoplasmic vacuoles. CONCLUSIONS: Immunohistochemical and ultrastructural results suggest that PGCGs of the jaws are composed mainly of cells of the mononuclear phagocyte system and that Langerhans cells are present in two thirds of the lesions.
Assuntos
Células Gigantes , Doenças da Gengiva/patologia , Granuloma de Células Gigantes/patologia , Actinas/análise , Anticorpos Monoclonais , Antígenos CD/análise , Antígenos de Diferenciação Mielomonocítica/análise , Células Gigantes/química , Células Gigantes/patologia , Células Gigantes/ultraestrutura , Humanos , Técnicas Imunoenzimáticas , Células de Langerhans , Antígenos Comuns de Leucócito/análise , Microscopia Eletrônica , Muramidase/análise , Fagócitos , Estudos Retrospectivos , Proteínas S100/análise , Vimentina/análise , alfa 1-Antiquimotripsina/análise , Fator de von Willebrand/análiseRESUMO
The infrared spectrum of native black-eyed pea trypsin and chymotrypsin inhibitor (BTCI) in solid film was measured from 550 to 1750 cm-1 and amide I-III, and V regions have been analyzed. By comparison between the observed bands with the modes calculated for several structures (available in the literature), the occurrence in BTCI of unordered, antipatallel beta-sheet, and beta-turn structures is suggested.
Assuntos
Fabaceae , Plantas Medicinais , Inibidores da Tripsina/análise , alfa 1-Antiquimotripsina/análise , Conformação Proteica , Espectrofotometria InfravermelhoRESUMO
The infrared spectrum of native black-eyed pea trypsin and chymotrypsin inhibitor (BTCI) in solid film was measured from 550 to 1750 cm-1 and amide I-III, and V regions have been analyzed. By comparison between the observed bands with the modes calculated for several structures (available inthe literature), the occurrence in BTCI of unordered, antipatallel ß-sheet, and ß-turn structures is suggested